In Vivo Investigation on the Effects of Metal Oxides in Hydroxyapatite Biocomposite Implants for Improving Bone Regeneration.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biological Trace Element Research Pub Date : 2025-05-16 DOI:10.1007/s12011-025-04651-9

Mostafa Mabrouk, Hanan H Beherei, Omar H Abd-Elkader, Fariborz Sharifianjazi, Diganta B Das

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引用次数: 0

Abstract

Incorporating biocompatible nanomaterials in bone implants continues to pose a significant challenge in biomedical engineering. In addressing this issue, this study examines the critical role of the interactions of nanoscale metal oxides, specifically Al₂O₃, TiO₂, and SiO₂, with hydroxyapatite while developing novel multiphase biocomposites. The study also investigates how animal models respond to these new implants while focusing on improving the physicochemical properties, microstructure and osteoconductivity of calcium phosphate ceramics. Blood antioxidant enzymes and bone turnover markers were examined, and the effects on vital organs like the kidneys and liver were investigated. The results demonstrated that hydroxyapatite/SiO₂/TiO₂ (SHT) composite significantly impacted the liver and kidney functions during the study periods. However, the hydroxyapatite/SiO₂/Al₂O₃ (SHA) composite had no measurable effect at 2 days, while there was a significant effect at 20 days. Overall, it is concluded that the SHT composite ceramic has more osteoconductive effects and relatively high toxicity on the liver and kidney. SHA is found to be moderately toxic, and hydroxyapatite/SiO₂ (SH) composite displayed lesser or no toxicity. The results, which showed a significant depletion of blood GPX and SOD, indicate that oxidative stress mediates the cytotoxicity of the SHT and SHA composites.

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羟基磷灰石生物复合种植体中金属氧化物促进骨再生的体内研究。

将生物相容性纳米材料整合到骨植入物中仍然是生物医学工程中的一个重大挑战。为了解决这一问题，本研究在开发新型多相生物复合材料的同时，研究了纳米级金属氧化物（特别是Al2O3、TiO2和SiO2）与羟基磷灰石相互作用的关键作用。该研究还研究了动物模型对这些新型植入物的反应，同时着重于改善磷酸钙陶瓷的物理化学性质，微观结构和骨导电性。检测血液抗氧化酶和骨转换标志物，并研究其对肾脏和肝脏等重要器官的影响。结果表明，羟基磷灰石/SiO2/TiO2 （SHT）复合物在研究期间显著影响肝脏和肾脏功能。然而，羟基磷灰石/SiO2/Al2O3 （SHA）复合材料在第2天没有明显的效果，而在第20天有显著的效果。综上所述，SHT复合陶瓷具有更强的骨传导作用，对肝脏和肾脏具有较高的毒性。SHA具有中等毒性，羟基磷灰石/SiO2 （SH）复合材料毒性较小或没有毒性。结果显示，血液中GPX和SOD明显减少，表明氧化应激介导了SHT和SHA复合材料的细胞毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biological Trace Element Research 生物-内分泌学与代谢

CiteScore

8.70

自引率

10.30%

发文量

459

审稿时长

2 months

期刊介绍： Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.