Genetic and Pathogenic Characterization of a Porcine Deltacoronavirus Strain Isolated in Zhejiang Province, China

IF 3.5 2区农林科学 Q2 INFECTIOUS DISEASES

Transboundary and Emerging Diseases Pub Date : 2025-05-18 DOI:10.1155/tbed/4084814

Ya-Qing Zhang, Bin Wang, Weiqun Wei, Wan Lu, En-Zhong Du, Yan Liu, Yong-Le Yang, Yao-Wei Huang

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引用次数: 0

Abstract

Porcine deltacoronavirus (PDCoV) has emerged as a significant pathogen in swine, affecting animal health and posing potential risks for cross-species transmission. In this study, we successfully isolated a PDCoV strain named HZYH-2019 from the feces of diarrheal sows in Zhejiang Province, China. The viral growth curve demonstrated strong adaptation of this strain to cells, with particularly high replication efficiency observed in LLC-PK1 cells. Genomic analysis revealed a high degree of nucleotide sequence similarity between PDCoV HZYH-2019 and other PDCoV strains. A notable mutation at the tenth amino acid position of the spike protein altered the predicted signal peptide position. Phylogenetic analyses indicated that PDCoV HZYH-2019 clustered with Chinese strains, while four Chinese strains were grouped within the American spectrum, suggesting that the pork trade may facilitate cross-border virus transmission. Analysis of known PDCoV strains inferred that PDCoV may have originated in Asia and that there is cross-species transmission from birds to mammals. Notably, PDCoV HZYH-2019 caused diarrhea in piglets without mortality, although significant intestinal lesions were observed. These findings enhance our understanding of PDCoV’s biological behavior and zoonotic potential, informing the development of effective vaccines and control measures to manage future outbreaks.

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浙江省猪三角冠状病毒分离株的遗传和致病性分析

猪三角冠状病毒（PDCoV）已成为猪的重要病原体，影响动物健康并具有跨物种传播的潜在风险。本研究成功从浙江省腹泻母猪粪便中分离出一株PDCoV，命名为HZYH-2019。病毒生长曲线显示了该菌株对细胞的强适应性，在LLC-PK1细胞中观察到特别高的复制效率。基因组分析显示，HZYH-2019与其他PDCoV毒株核苷酸序列高度相似。刺突蛋白第10个氨基酸位置的显著突变改变了预测的信号肽位置。系统发育分析表明，PDCoV HZYH-2019与中国毒株聚集在一起，而4个中国毒株属于美国毒株，这表明猪肉贸易可能促进了病毒的跨境传播。对已知PDCoV毒株的分析推断，PDCoV可能起源于亚洲，并且存在从鸟类到哺乳动物的跨物种传播。值得注意的是，PDCoV HZYH-2019引起仔猪腹泻，但没有死亡，尽管观察到明显的肠道病变。这些发现增强了我们对PDCoV的生物学行为和人畜共患潜力的理解，为开发有效的疫苗和控制措施提供了信息，以管理未来的疫情。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transboundary and Emerging Diseases 农林科学-传染病学

CiteScore

8.90

自引率

9.30%

发文量

350

审稿时长

1 months

期刊介绍： Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.