Immunoglobulin-derived peptide MEANP1 of human Milk in the protection against experimental necrotizing Enterocolitis via IκB/ NF-κB pathway

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-05-19 DOI:10.1016/j.jff.2025.106826

Shuwen Yao , Min Zhang , Qinlei Yu , Liping Xu , Xiangyun Yan , Wenjuan Chen , Chengyao Jiang , Yanjie Chen , Kedi Xi , Yu Dai , Shushu Li , Shuping Han , Xiaohui Chen

{"title":"Immunoglobulin-derived peptide MEANP1 of human Milk in the protection against experimental necrotizing Enterocolitis via IκB/ NF-κB pathway","authors":"Shuwen Yao , Min Zhang , Qinlei Yu , Liping Xu , Xiangyun Yan , Wenjuan Chen , Chengyao Jiang , Yanjie Chen , Kedi Xi , Yu Dai , Shushu Li , Shuping Han , Xiaohui Chen","doi":"10.1016/j.jff.2025.106826","DOIUrl":null,"url":null,"abstract":"<div><div>Necrotizing enterocolitis (NEC) is one of the most common intestinal emergencies in newborn infants. It is closely related to abnormal activation of intestinal inflammation and impaired barrier function. Studies have shown that breast milk exosomes can effectively improve NEC, but the specific mechanism remains unclear. Human breast milk exosomal cargoes are emerging as new modulators for NEC. To evaluate the function and potential mechanisms of action of peptide MEANP1 both in vivo and in vitro. MEANP1 was found to improve the survival rate, abdominal distension and hematochezia in NEC models. Intestinal histological damage was improved, along with decreased expression of pro-inflammatory factors and increased expression of intestinal barrier protein. In IEC-6 cells, MEANP1 can improve LPS-induced inhibition of proliferation, migration and barrier damage. The pro-inflammatory factors TLR4, TNF-α and IL-1β were inhibited by MEANP1. We verified by RNA-seq technique and rescue experiment that it can inhibit the phosphorylation of NF-κB by inhibiting the phosphorylation of IκBα to protect from NEC. MEANP1 is expected to be a treatment for NEC.</div></div>","PeriodicalId":360,"journal":{"name":"Journal of Functional Foods","volume":"129 ","pages":"Article 106826"},"PeriodicalIF":3.8000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Functional Foods","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1756464625001689","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Necrotizing enterocolitis (NEC) is one of the most common intestinal emergencies in newborn infants. It is closely related to abnormal activation of intestinal inflammation and impaired barrier function. Studies have shown that breast milk exosomes can effectively improve NEC, but the specific mechanism remains unclear. Human breast milk exosomal cargoes are emerging as new modulators for NEC. To evaluate the function and potential mechanisms of action of peptide MEANP1 both in vivo and in vitro. MEANP1 was found to improve the survival rate, abdominal distension and hematochezia in NEC models. Intestinal histological damage was improved, along with decreased expression of pro-inflammatory factors and increased expression of intestinal barrier protein. In IEC-6 cells, MEANP1 can improve LPS-induced inhibition of proliferation, migration and barrier damage. The pro-inflammatory factors TLR4, TNF-α and IL-1β were inhibited by MEANP1. We verified by RNA-seq technique and rescue experiment that it can inhibit the phosphorylation of NF-κB by inhibiting the phosphorylation of IκBα to protect from NEC. MEANP1 is expected to be a treatment for NEC.

Abstract Image

查看原文本刊更多论文

人乳免疫球蛋白衍生肽MEANP1通过i -κB / NF-κB途径对实验性坏死性小肠结肠炎的保护作用

坏死性小肠结肠炎（NEC）是新生儿最常见的肠道急症之一。它与肠道炎症异常激活和屏障功能受损密切相关。研究表明，母乳外泌体可以有效改善NEC，但具体机制尚不清楚。人类母乳外泌体货物正在成为NEC的新调节剂。目的：探讨多肽MEANP1在体内和体外的功能及其可能的作用机制。发现MEANP1能提高NEC模型的存活率、腹胀和便血率。肠道组织损伤得到改善，促炎因子表达减少，肠屏障蛋白表达增加。在IEC-6细胞中，MEANP1可以改善lps诱导的增殖、迁移和屏障损伤的抑制。MEANP1可抑制促炎因子TLR4、TNF-α和IL-1β。我们通过RNA-seq技术和救援实验证实，它可以通过抑制i -κB α的磷酸化来抑制NF-κB的磷酸化，从而保护NEC。MEANP1有望成为NEC的一种治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.