PalCYCD3;3 breaks axillary bud dormancy and promotes shoot branching through activation by PalBES1 in the BR signaling pathway

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-09 DOI:10.1016/j.plaphy.2025.109993

Zhao-qun Wu , Ye-Bo Yang , Xiu-Xing Zhang , Shi-Yi Wang , Yu-Wen Wang , Jing Xue , Yue-Xuan Zhang , Meng-Yu Gai , Bo-Hao Duan , Hai-Ling Yang

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引用次数: 0

Abstract

The development of lateral branches in plants is intricately modulated by phytohormonal signaling networks; however, the functional role of D-type cyclins (CYCDs) within this regulatory scheme is not yet fully elucidated. Recent investigations have identified brassinosteroids (BRs) as pivotal regulators of cell cycle dynamics, yet their interactions with CYCD-mediated pathways in meristematic activity require systematic investigation. This research aimed to clarify the function of PalCYCD3; 3 in the regulation of axillary meristem (AM) and its interaction with BR signaling in Populus alba. Histochemical GUS staining demonstrated the specific localization of PalCYCD3;3 within the organizing centers of both the shoot apical meristem (SAM) and AM. PalCYCD3;3-overexpressing P. alba displayed a significant increase in lateral branch formation compared to wild-type counterparts, alongside a marked reduction in AM cell size, mirroring the effects observed in wild-type plants treated with 1 μM brassinolide (BL). Treatment with BL was shown to upregulate both PalCYCD3;3 and the BR signaling mediator PalBES1 in axillary buds. Dual-luciferase reporter assays and electrophoretic mobility shift assays verified the direct interaction of PalBES1 with the promoter of PalCYCD3;3. Additionally, yeast two-hybrid screening combined with bimolecular fluorescence complementation confirmed the physical associations between PalCYCD3; 3 and key cell cycle regulators PalCDKA; 1, PalCDKB1; 1, PalCDKD; 1, and PalCDKE; 1. Our findings establish that BR signaling activates PalCYCD3; 3-CDK complexes through PalBES1-mediated transcriptional regulation, thereby promoting AM cell proliferation and lateral branch development.

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PalCYCD3；3通过BR信号通路中PalBES1的激活，打破腋芽休眠，促进芽枝分枝

植物侧枝的发育受植物激素信号网络的复杂调控；然而，d型细胞周期蛋白（CYCDs）在这一调控机制中的功能作用尚未完全阐明。最近的研究已经确定油菜素内酯（BRs）是细胞周期动力学的关键调节因子，但它们与cycd介导的分生组织活性途径的相互作用需要系统的研究。本研究旨在阐明PalCYCD3的功能；3在白杨腋窝分生组织（axillary merisystem， AM）调控及其与BR信号的相互作用中的作用。组织化学GUS染色显示PalCYCD3特异性定位于芽顶分生组织（SAM）和芽顶分生组织（AM）的组织中心。与野生型相比，过表达PalCYCD3；3的P. alba的侧枝形成显著增加，AM细胞大小显著减小，这与在1 μM油菜素内酯（BL）处理的野生型植物中观察到的效果一致。结果显示，BL处理可上调腋芽中PalCYCD3；3和BR信号介质PalBES1。2 .双荧光素酶报告基因试验和电泳迁移率转移试验证实PalBES1与PalCYCD3启动子直接相互作用；此外，酵母双杂交筛选结合双分子荧光互补证实了PalCYCD3；3和关键细胞周期调节因子PalCDKA；1、PalCDKB1;1、PalCDKD;1、PalCDKE；1. 我们的研究结果表明，BR信号激活PalCYCD3；3-CDK复合物通过palbes1介导的转录调控，从而促进AM细胞增殖和侧支发育。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.