Comparative transcriptome analysis reveals aggravated damage on rice plants by brown planthoppers under elevated CO2

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-14 DOI:10.1016/j.plaphy.2025.109998

Yanhui Wang , Huirong Mai , Ruichuan Duan , Xiaowei Liu , Fajun Chen

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引用次数: 0

Abstract

The increase of atmospheric CO₂ concentration will inevitably affect the photosynthesis of C₃ plants, thus affecting herbivorous insects. Previous studies have shown that elevated CO₂ (eCO₂) may aggravate damage of brown planthopper (BPH) Nilaparvata lugens for rice plants. However, the molecular mechanism of this phenomenon is unclear. The comparative transcriptome analysis combined with corresponding phenotypic changes of rice plants and BPHs under ambient CO₂ (aCO₂) and eCO₂ were studied to reveal the molecular mechanism of aggravated damage. The contents of soluble sugar and free fatty acid in rice stems were significantly increased while total amino acid was significantly decreased under eCO₂ in contrast to aCO₂. Besides, compared with aCO₂, eCO₂ weakened the secondary defense of rice plants against BPH-damage. More importantly, the contents of trehalose, glucose and triglycerides were increased and total protein and total amino acid were decreased in BPHs fed on rice plants grown under eCO₂, resulting in stronger energy metabolism, thus enhancing that feeding behavior to attain sufficient nutrients. Therefore, both the weaken of defense in rice plants and enhancement of piercing and sucking ability of BPHs were presumed to cause the aggravated damage on rice plants by BPHs under eCO₂.

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对比转录组分析表明，高浓度CO2环境下褐飞虱对水稻植株的危害加重

大气CO2浓度的增加必然会影响C3植物的光合作用，从而影响草食性昆虫。已有研究表明，CO2 （eCO2）升高可能加重褐飞虱（BPH）对水稻的危害。然而，这一现象的分子机制尚不清楚。通过比较转录组分析，结合环境CO2 （aCO2）和eCO2对水稻植株和BPHs的相应表型变化，揭示了加重损伤的分子机制。与aCO2处理相比，eCO2处理显著提高了水稻茎部可溶性糖和游离脂肪酸含量，显著降低了总氨基酸含量。此外，与aCO2相比，eCO2削弱了水稻对bph伤害的次生防御能力。更重要的是，在eCO2条件下，BPHs取食水稻植株时，海藻糖、葡萄糖和甘油三酯含量升高，总蛋白和总氨基酸含量降低，导致能量代谢增强，从而增强了取食行为，以获取足够的营养。因此，可以推测，在eCO2条件下，bph对水稻植株的伤害加重是由于bph防御能力的减弱和刺吸能力的增强。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.