Engineering CRISPR/Cas12-based biosensors: Recent advances and future perspectives

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-05-13 DOI:10.1016/j.trac.2025.118296

Lijuan Yin , Caihong Zhou , Baoxue Gao , Fufeng Liu , Long Ma , Fei Guo

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引用次数: 0

Abstract

Rapid, sensitive, and accurate biosensing is instrumental for clinical diagnostics, food safety, and environmental monitoring. The CRISPR/Cas12 system has emerged as a robust biosensing tool, leveraging its biocompatibility, distinctive cleavage activity, and user-friendly design. In recent years, numerous CRISPR/Cas-based biosensing strategies have been developed to enhance detection sensitivity, specificity, and portability, significantly advanced the molecular diagnostics field. This review systematically discusses recent advances in various engineering strategies aimed at improving the performance of CRISPR/Cas12-based biosensors, with emphasis on their practical applications. Specifically, progresses are highlighted in optimizing Cas effector proteins, CRISPR RNAs, and reaction buffer formulations. Diverse signal output methodologies are compared including fluorescence, colorimetry, electrochemical sensing, surface-enhanced Raman spectroscopy, and deep-learning-assisted detection techniques. Finally, challenges and future prospectives of CRISPR/Cas12-based biosensing technologies are discussed to help the development of optimal biosensing tools.

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基于CRISPR/ cas12的生物传感器工程：最新进展和未来展望

快速、灵敏、准确的生物传感是临床诊断、食品安全和环境监测的工具。CRISPR/Cas12系统已成为一种强大的生物传感工具，利用其生物相容性，独特的裂解活性和用户友好的设计。近年来，许多基于CRISPR/ cas的生物传感策略被开发出来，以提高检测的灵敏度、特异性和可移植性，极大地推动了分子诊断领域的发展。本文系统地讨论了旨在提高基于CRISPR/ cas12的生物传感器性能的各种工程策略的最新进展，重点介绍了它们的实际应用。具体而言，在优化Cas效应蛋白、CRISPR rna和反应缓冲制剂方面取得了进展。比较了不同的信号输出方法，包括荧光、比色法、电化学传感、表面增强拉曼光谱和深度学习辅助检测技术。最后，讨论了基于CRISPR/ cas12的生物传感技术的挑战和未来展望，以帮助开发最佳的生物传感工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.