Development of chamber-based digital polymerase chain reaction: From chip to device

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-05-13 DOI:10.1016/j.trac.2025.118299

Jizhi Tong , Fangfang Yin , Mengda Liang , Wen Zong , Yu Wang , Duo Xiao , Juxin Yin , Ying Mu

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引用次数: 0

Abstract

Digital polymerase chain reaction (dPCR) is a powerful tool for nucleic acid detection. Chamber-based digital polymerase chain reaction (cdPCR), a specific implementation of dPCR, offers advantages such as high stability, enhanced accuracy, and improved chamber utilization. Despite its advantages, a comprehensive review of cdPCR remains lacking. This review summarizes cutting-edge research on cdPCR chips, equipment, and related Artificial intelligence (AI) image analysis algorithms. It introduces the structure and function of various cdPCR digital chips, including the integrated fluidic circuits (IFC) cdPCR chip, the microwell array cdPCR chip (lacking microchannel), and the SlipChip. Additionally, it highlights recent advancements in the mechanical design of cdPCR devices and enhancements in image processing techniques. Finally, the review concludes by summarizing the opportunities and key challenges in cdPCR, offering insights into prospects critical for the continued progress of this technology.

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基于腔室的数字聚合酶链反应的发展：从芯片到设备

数字聚合酶链反应（dPCR）是一种强大的核酸检测工具。基于腔室的数字聚合酶链反应（cdPCR）是dPCR的一种具体实现，具有高稳定性、高准确性和提高腔室利用率等优点。尽管cdPCR具有诸多优势，但目前仍缺乏对其的全面综述。本文综述了cdPCR芯片、设备和相关人工智能（AI）图像分析算法的最新研究进展。介绍了各种cdPCR数字芯片的结构和功能，包括集成流控电路（IFC） cdPCR芯片、微孔阵列cdPCR芯片（无微通道）和SlipChip。此外，它还强调了cdPCR装置机械设计的最新进展和图像处理技术的增强。最后，综述总结了cdPCR的机遇和主要挑战，并对该技术的持续发展提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.