Integrated multi-omics and DNA stable-isotope probing approaches to reveal soil-ryegrass response to ionic rare earth mineral ammonium-lead contamination

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-05-18 DOI:10.1016/j.jhazmat.2025.138658

Huixian Yang, Jing Zhou, Jun Zhou

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Abstract

The extensive use of ammonium (NH₄⁺) sulfate in ionic rare earth mining has resulted in soil contamination with NH₄⁺ and lead (Pb), posing significant challenges for ecological restoration. Here, multi-omics and DNA stable-isotope probing (DNA-SIP) approaches were utilized to investigate soil nitrogen cycling and the molecular response of ryegrass (Lolium perenne L.) to NH₄⁺ (180-720 mg kg^-1)-Pb²⁺ (207-828 mg kg^-1) co-contamination. A synergistic interaction between NH₄⁺ and Pb²⁺ was observed, significantly inhibited ryegrass growth, and induced oxidative stress and mitochondrial swelling. The EC₅₀ toxicity thresholds were 383 mg kg⁻¹ for NH₄⁺ and 512 mg kg⁻¹ for Pb. The Integrated Biomarker Response (IBRv2) model elucidated the synergistic toxic effects. Transcriptomic and metabolomic analyses indicated that ryegrass roots enhanced carbon metabolism and antioxidant response pathways related to stress tolerance. Galactose metabolism and lysine degradation were identified as key pathways associated with stress response. Co-contamination with NH₄⁺ and Pb²⁺ reduced ryegrass root ¹⁵N-total nitrogen (TN) by 30% while increasing soil ¹⁵N-NH₄⁺ residue by 95% and decreasing ¹⁵N-microbial biomass nitrogen (MBN) by 59%, compared to NH₄⁺ single contamination. DNA-SIP analysis revealed that ryegrass cultivation under NH₄⁺- Pb²⁺ co-contamination increased the abundance of plant growth-promoting rhizobacteria (Dyella), acid-tolerant nitrogen (Acidibacter), and sulfur-cycling taxa (Desulfosporosinus). The presence of raffinose and chlorogenic acid in ryegrass root metabolites was associated with shifts in the structure and composition of using NH₄⁺ active microbial taxa. These findings provide valuable insights into plant-soil-microbe interactions under multi-pollutant stress and offer practical strategies for phytoremediation and ecological restoration in areas affected by mining.

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综合多组学和DNA稳定同位素探测方法揭示土壤-黑麦草对离子型稀土矿物铵铅污染的响应

离子型稀土开采中大量使用硫酸铵，造成土壤NH4+和铅污染，给生态恢复带来重大挑战。采用多组学和DNA稳定同位素探测（DNA- sip）方法研究了黑麦草（Lolium perenne L.）土壤氮循环及对NH4+ (180 ~ 720 mg kg-1)-Pb2+ (207 ~ 828 mg kg-1)共污染的分子响应。NH4+和Pb2+之间存在协同作用，显著抑制黑麦草生长，诱导氧化应激和线粒体肿胀。EC50毒性阈值NH4+为383 mg kg⁻¹，Pb为512 mg kg⁻¹。综合生物标志物反应（IBRv2）模型阐明了协同毒性作用。转录组学和代谢组学分析表明，黑麦草根增强了碳代谢和抗氧化反应途径，与胁迫耐受有关。半乳糖代谢和赖氨酸降解被确定为与应激反应相关的关键途径。与单一NH4+污染相比，NH4+和Pb2+共污染使黑麦草根系15n -总氮（TN）减少30%，土壤15N-NH4+残留量增加95%，15n -微生物生物量氮（MBN）减少59%。DNA-SIP分析显示，在NH4+- Pb2+共污染条件下，黑麦草中促进植物生长的根瘤菌（Dyella）、耐酸氮菌（Acidibacter）和硫循环类群（Desulfosporosinus）的丰度增加。黑麦草根代谢产物中棉子糖和绿原酸的存在与利用NH4+的活性微生物类群的结构和组成变化有关。这些发现为多污染物胁迫下植物-土壤-微生物的相互作用提供了有价值的见解，并为受采矿影响地区的植物修复和生态恢复提供了实用的策略。

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.