Time-Dependent EDTA Effect on Leukocyte Differential Count and Morphology Compared to Blood Smear Made by Direct Finger Prick

IF 2.3 4区医学 Q3 HEMATOLOGY

International Journal of Laboratory Hematology Pub Date : 2025-05-16 DOI:10.1111/ijlh.14498

Anna Irding, Filip Landgren, Elisabeth Aardal

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引用次数: 0

Abstract

Introduction

The impact of ethylenediaminetetraacetic acid (EDTA) on leukocyte count and morphology has led to the current recommendation that smears made from EDTA blood should be prepared within 4 h of sampling. However, previous studies have only been performed on smears with normal cell counts, and smears made from K₂EDTA blood have not been studied in comparison with smears made from direct finger prick. The aim of this study was to investigate the time limit for performing a morphological assessment on smears from EDTA blood without compromising the results.

Methods

Blood samples from 37 patients with known or suspected abnormal leukocyte morphology were selected, with smears from direct finger prick used as controls. Smears were prepared from the original EDTA tube every 4 h up to 24 h after sampling. The smears were evaluated for differential count, morphological assessment, and smear quality scoring.

Results

No significant difference in cell count was observed on smears made up to 12 h after sampling for neutrophils (p = 0.430), lymphocytes (p = 0.080), monocytes (p = 0.948) eosinophils (p = 0.398), basophils (p = 0.460), and blast cells (p = 0.239) compared to smears made by finger prick. However, smears prepared later than 8 h postsampling showed a significant increase in morphological changes.

Conclusion

A manual differential leukocyte count can be reliably performed on smears from EDTA blood within 12 h, whereas a comprehensive morphological assessment requires smears to be prepared within 8 h of sampling.

Abstract Image

查看原文本刊更多论文

EDTA对直接手指刺血涂片白细胞计数和形态学的影响。

简介：乙二胺四乙酸（EDTA）对白细胞计数和形态学的影响，导致目前建议从EDTA血液中提取的涂片应在取样后4小时内制备。然而，先前的研究仅对细胞计数正常的涂片进行了研究，并且尚未将K2EDTA血液涂片与直接刺破手指的涂片进行比较。本研究的目的是研究在不影响结果的情况下对EDTA血液涂片进行形态学评估的时间限制。方法：选取已知或疑似白细胞形态异常的患者37例，以手指直接穿刺涂片为对照。从原始EDTA管中每4小时制备一次涂片，直至取样后24小时。对涂片进行鉴别计数、形态学评估和涂片质量评分。结果：取样后12 h涂片中中性粒细胞（p = 0.430）、淋巴细胞（p = 0.080）、单核细胞（p = 0.948）、嗜酸性粒细胞（p = 0.398）、嗜碱性粒细胞（p = 0.460）和胚细胞（p = 0.239）的细胞计数与针刺手指涂片比较无显著差异。然而，采样后8小时后制备的涂片显示形态学变化明显增加。结论：人工鉴别白细胞计数可以在12小时内可靠地对EDTA血液涂片进行检测，而全面的形态学评估需要在取样后8小时内制备涂片。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Laboratory Hematology 医学-血液学

CiteScore

4.50

自引率

6.70%

发文量

211

审稿时长

6-12 weeks

期刊介绍： The International Journal of Laboratory Hematology provides a forum for the communication of new developments, research topics and the practice of laboratory haematology. The journal publishes invited reviews, full length original articles, and correspondence. The International Journal of Laboratory Hematology is the official journal of the International Society for Laboratory Hematology, which addresses the following sub-disciplines: cellular analysis, flow cytometry, haemostasis and thrombosis, molecular diagnostics, haematology informatics, haemoglobinopathies, point of care testing, standards and guidelines. The journal was launched in 2006 as the successor to Clinical and Laboratory Hematology, which was first published in 1979. An active and positive editorial policy ensures that work of a high scientific standard is reported, in order to bridge the gap between practical and academic aspects of laboratory haematology.