Nutrients and phytochemicals characterisations, acute and sub-acute oral toxicity studies of BobyGuard C, a polyherbal nutraceutical with anti-breast cancer properties.

IF 3.6 Q2 TOXICOLOGY
Frontiers in toxicology Pub Date : 2025-05-01 eCollection Date: 2025-01-01 DOI:10.3389/ftox.2025.1598185
Borelle Mafogang, Roger Ponka, Joseph Ngakou Mukam, Elie Fokou
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引用次数: 0

Abstract

Background: In 2022, approximately 2.3 million new cases of female breast cancer and 670,000 related deaths worldwide despite significant advancements in conventional treatments. BobyGuard C (BGC) is a novel polyherbal nutraceutical formulated from five plants, selected for their antioxidant, anticancer, anti-inflammatory and nutritional properties to be used for breast cancer management. This study aimed to characterize its physicochemical, nutritional, and phytochemical properties as well as assess its safety through acute and sub-acute oral toxicity studies in Wistar rats.

Methods: Thecomposition of BGC was analyzed for macronutrients, minerals, and phytochemicals using standard methods. Antioxidant activity was assessed through DPPH, TAC and FRAP assays, while antiproliferative activity was evaluated using the MTT assay on MDA-MB 231 and MCF-7 breast cancer cell lines. Acute (single 5,000 mg/kg dose with 14 days observation) and sub-acute oral (daily administration of 784, 1,568, and 3,136 mg/kg for 28 days) toxicity studies in female Wistar rats followed OECD guidelines.

Results: BGC was found to be rich in proteins (38.36 g/100 g), carbohydrates (59.70 g/100 g), and essential minerals such as magnesium (60,066.67 µg/100 g), and it was free from toxic heavy metals. Several bioactive compounds, including diosgenin, diosbulbin H, β-carotene, Bafoudiosbulbin G and catechin were identified in BGC. Phytochemical analysis revealed high levels of phenols (9,783.48 mg GAE/100 g), flavonoids (47.72 mg QuE/100 g), and alkaloids (106.14 mg berberine eq/100 g), contributing to its strong antioxidant activity (DPPH inhibition: 90.39%). BGC exhibited significant antiproliferative effects on MDA-MB 231 cells, highlighting its potential anticancer activity. Acute toxicity tests showed no mortality at 5,000 mg/kg, with an LD50 exceeding this dose. In the sub-acute 28-day repeated-dose oral study, doses up to 3,136 mg/kg/day resulted in some dose dependent hematological and biochemical changes but no histopathological abnormalities were observed indicating its safety at lower doses.

Conclusion: BGC is a nutritionally rich formulation with potent antioxidant and anticancer potential, demonstrating a favorable safety profile at lower dose (784 mg/kg).

BobyGuard C是一种具有抗乳腺癌特性的多草药营养保健品,其营养成分和植物化学物质特征、急性和亚急性口服毒性研究。
背景:2022年,尽管传统治疗取得了重大进展,但全球仍有约230万新发女性乳腺癌病例和67万相关死亡病例。BobyGuard C (BGC)是一种新型的多草药营养保健品,由五种植物配制而成,因其抗氧化、抗癌、抗炎和营养特性而被选中用于乳腺癌的治疗。本研究旨在通过Wistar大鼠急性和亚急性口服毒性研究,表征其理化、营养和植物化学特性,并评估其安全性。方法:采用标准方法分析BGC中常量营养素、矿物质和植物化学物质的组成。通过DPPH、TAC和FRAP测定抗氧化活性,通过MTT测定MDA-MB 231和MCF-7乳腺癌细胞株的抗增殖活性。雌性Wistar大鼠急性(单次给药5000 mg/kg,观察14天)和亚急性口服(每天给药784、1568和3136 mg/kg,观察28天)毒性研究遵循经合组织指南。结果:BGC富含蛋白质(38.36 g/100 g)、碳水化合物(59.70 g/100 g)和镁等必需矿物质(60,066.67µg/100 g),不含有毒重金属。鉴定出薯蓣皂苷元、薯蓣皂苷H、β-胡萝卜素、薯蓣皂苷G和儿茶素等多种生物活性成分。植物化学分析表明,其酚类物质(9783.48 mg GAE/100 g)、类黄酮(47.72 mg QuE/100 g)和生物碱(106.14 mg小檗碱eq/100 g)含量较高,具有较强的抗氧化活性(DPPH抑制率为90.39%)。BGC对MDA-MB 231细胞有明显的抗增殖作用,显示其潜在的抗癌活性。急性毒性试验表明,5,000 mg/kg剂量下无死亡,致死剂量50超过该剂量。在亚急性28天重复给药口服研究中,高达3136 mg/kg/天的剂量导致一些剂量依赖性血液和生化变化,但未观察到组织病理学异常,表明其在低剂量下是安全的。结论:BGC是一种营养丰富的制剂,具有较强的抗氧化和抗癌潜力,在低剂量(784 mg/kg)下具有良好的安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.80
自引率
0.00%
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审稿时长
13 weeks
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