P P Barbosa, D M Leme, N G Motta, W L E Magalhães, J L Proenca-Modena, J-Y Maillard
{"title":"Exploring the bactericidal efficacy of a new potassium monopersulphate-based disinfectant.","authors":"P P Barbosa, D M Leme, N G Motta, W L E Magalhães, J L Proenca-Modena, J-Y Maillard","doi":"10.1016/j.jhin.2025.04.033","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Staphylococcus aureus and Klebsiella pneumoniae are common pathogens responsible for hospital-acquired infections. Both species can survive on surfaces following desiccation and form dry surface biofilms (DSB), which complicates the disinfection process.</p><p><strong>Aim: </strong>To evaluate the efficacy of an innovative potassium monopersulphate-based nanotechnology formulation (MPS) against both planktonic and sessile S. aureus and K. pneumoniae.</p><p><strong>Methods: </strong>The bactericidal efficacy of MPS was tested in comparison with sodium hypochlorite (NaOCl) and didecyldimethylammonium chloride (DDAC), which served as controls. The assessment was performed against planktonic bacteria, hydrated biofilm, and dry surface biofilm (DSB) using standard suspension and carrier tests. Scanning electron microscopy (SEM) was employed to identify any gross structural damage.</p><p><strong>Findings: </strong>MPS (2% w/v) achieved a ≥4 log<sub>10</sub> reduction in K. pneumoniae with a short contact time, regardless of the test protocol. S. aureus proved more resilient, but the introduction of wiping reduced the contact time needed to achieve a 4 log<sub>10</sub> reduction from 15 to 5 minutes. SEM analysis revealed gross structural damage in both species following MPS treatment. The other disinfectants tested were also bactericidal, achieving ≥4 log<sub>10</sub> reduction within 1-5 minutes, with the exception of DDAC against hydrated biofilms.</p><p><strong>Conclusions: </strong>The potassium monopersulphate-based formulation was found to be an effective bactericide, including against dry surface biofilms (DSB). Its efficacy compares favourably with other biocides commonly used in healthcare settings, and its biodegradability makes it a promising candidate for further development. However, optimisation of the mechanical removal process will be essential to enhance MPS efficacy in practical applications.</p>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":" ","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hospital Infection","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jhin.2025.04.033","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Staphylococcus aureus and Klebsiella pneumoniae are common pathogens responsible for hospital-acquired infections. Both species can survive on surfaces following desiccation and form dry surface biofilms (DSB), which complicates the disinfection process.
Aim: To evaluate the efficacy of an innovative potassium monopersulphate-based nanotechnology formulation (MPS) against both planktonic and sessile S. aureus and K. pneumoniae.
Methods: The bactericidal efficacy of MPS was tested in comparison with sodium hypochlorite (NaOCl) and didecyldimethylammonium chloride (DDAC), which served as controls. The assessment was performed against planktonic bacteria, hydrated biofilm, and dry surface biofilm (DSB) using standard suspension and carrier tests. Scanning electron microscopy (SEM) was employed to identify any gross structural damage.
Findings: MPS (2% w/v) achieved a ≥4 log10 reduction in K. pneumoniae with a short contact time, regardless of the test protocol. S. aureus proved more resilient, but the introduction of wiping reduced the contact time needed to achieve a 4 log10 reduction from 15 to 5 minutes. SEM analysis revealed gross structural damage in both species following MPS treatment. The other disinfectants tested were also bactericidal, achieving ≥4 log10 reduction within 1-5 minutes, with the exception of DDAC against hydrated biofilms.
Conclusions: The potassium monopersulphate-based formulation was found to be an effective bactericide, including against dry surface biofilms (DSB). Its efficacy compares favourably with other biocides commonly used in healthcare settings, and its biodegradability makes it a promising candidate for further development. However, optimisation of the mechanical removal process will be essential to enhance MPS efficacy in practical applications.
期刊介绍:
The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience.
The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that:
provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings;
provide new insight into cleaning, disinfection and decontamination;
provide new insight into the design of healthcare premises;
describe novel aspects of outbreaks of infection;
throw light on techniques for effective antimicrobial stewardship;
describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control;
improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change;
improve understanding of the use of IT systems in infection surveillance and prevention and control.