Intestinal microbiota profiles of captive-bred cynomolgus macaques reveal influence of biogeography and age.

IF 4.9 Q1 MICROBIOLOGY
C Purse, A Parker, S A James, D J Baker, C J Moss, R Evans, J Durham, S G P Funnell, S R Carding
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引用次数: 0

Abstract

Background: Age-associated changes to the intestinal microbiome may be linked to inflammageing and the development of age-related chronic diseases. Cynomolgus macaques, a common animal model in biomedical research, have strong genetic physiological similarities to humans and may serve as beneficial models for the effect of age on the human microbiome. However, age-associated changes to their intestinal microbiome have previously only been investigated in faecal samples. Here, we have characterised and investigated the effects of age in the cynomolgus macaque intestinal tract in luminal samples from both the small and large intestine.

Results: Whole metagenomic shotgun sequencing was used to analyse the microbial communities in intestinal content obtained from six different intestinal regions, covering the duodenum to distal colon, of 24 healthy, captive-bred cynomolgus macaques, ranging in age from 4 to 20 years. Both reference-based and assembly-based computational profiling approaches were used to analyse changes to intestinal microbiota composition and metabolic potential associated with intestinal biogeography and age. Reference-based computational profiling revealed a significant and progressive increase in both species richness and evenness along the intestinal tract. The microbial community composition also significantly differed between the small intestine, caecum, and colon. Notably, no significant changes in the taxonomic abundance of individual taxa with age were found except when sex was included as a covariate. Additionally, using an assembly-based computational profiling approach, 156 putative novel bacterial and archaeal species were identified.

Conclusions: We observed limited effects of age on the composition of the luminal microbiota in the profiled regions of the intestinal tract except when sex was included as a covariate. The enteric microbial communities of the small and the large intestine were, however, distinct, highlighting the limitations of frequently used faecal microbial profiling as a proxy for the intestinal microbiota. The identification of a number of putative novel microbial taxa contributes to knowledge of the full diversity of the cynomolgus macaque intestinal microbiome.

人工饲养食蟹猕猴肠道菌群特征揭示了生物地理和年龄的影响。
背景:肠道微生物组的年龄相关变化可能与炎症和年龄相关慢性疾病的发展有关。食蟹猕猴是生物医学研究中常用的动物模型,与人类具有很强的遗传生理相似性,可以作为年龄对人类微生物组影响的有益模型。然而,与年龄相关的肠道微生物组变化以前只在粪便样本中进行过研究。在这里,我们对食蟹猴小肠和大肠的肠道样本中年龄的影响进行了表征和研究。结果:采用全宏基因组霰弹枪测序方法,对24只健康的圈养食蟹猕猴肠道内容物中的微生物群落进行了分析,这些肠道内容物来自6个不同的肠道区域,从十二指肠到结肠远端,年龄从4岁到20岁不等。基于参考文献和基于装配的计算分析方法被用于分析肠道微生物群组成和代谢潜力与肠道生物地理和年龄相关的变化。基于参考的计算分析显示,沿着肠道的物种丰富度和均匀度都显着逐渐增加。小肠、盲肠和结肠的微生物群落组成也存在显著差异。值得注意的是,除了将性别作为协变量外,单个分类群的分类丰度没有发现年龄的显著变化。此外,使用基于装配的计算分析方法,鉴定了156种假定的新型细菌和古细菌。结论:我们观察到年龄对肠道剖面区域内肠道微生物群组成的影响有限,除非将性别作为协变量考虑在内。然而,小肠和大肠的肠道微生物群落是不同的,这突出了经常使用粪便微生物谱作为肠道微生物群代理的局限性。一些假定的新微生物分类群的鉴定有助于了解食蟹猴肠道微生物群的全部多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
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审稿时长
13 weeks
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