LncRNA PGM5-AS1 regulates cell functions and acts as a potential biomarker to predict prognosis in breast cancer.

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Journal of Biological Markers Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI:10.1177/03936155251325846

Lei Xiang, Yueqing Yin, Linna Kong, Pengfei Li, Huihui Zhang, Wenqing Bian, Zhaoxu Wu

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引用次数: 0

Abstract

BackgroundThe aggressive proliferation and spread of breast cancer contributes to a dismal clinical outcome. The present study was to investigate the function and underlying mechanism of the long non-coding RNA (lncRNA) PGM5-AS1 in the modulation of breast cancer.MethodsQuantitative real time-polymerase chain reaction was utilized to assess the levels of PGM5-AS1 and miR-18a-3p. The prognostic significance was evaluated through Kaplan-Meier survival analysis and multivariate Cox regression analysis. Cell viability in breast cancer cells was measured utilizing a cell counting kit-8 kit. Cell migration and invasion were investigated using a transwell assay. The targeted regulatory interaction between PGM5-AS1, miR-18a-3p, and TGFBR3 was validated via dual luciferase reporter gene assay.ResultLevels of PGM5-AS1 were low in both breast tissue and cancer cell lines. This reduction in expression was linked to various clinical characteristics and a reduced overall survival rate in breast cancer patients. Upregulation of PGM5-AS1 expression noticeably inhibited proliferation, migration, and invasion in breast cancer cells. PGM5-AS1 regulated breast cancer development by controlling the miR-18a-3p/TGFBR3 axis.ConclusionThe lncRNA PGM5-AS1/miR-18a-3p/TGFBR3 axis is considered a potential genetic target for the development of breast cancer treatments.

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LncRNA PGM5-AS1调节细胞功能，作为预测乳腺癌预后的潜在生物标志物。

背景：乳腺癌的侵袭性增殖和扩散导致了令人沮丧的临床结果。本研究旨在探讨长链非编码RNA (lncRNA) PGM5-AS1在乳腺癌调控中的作用及其潜在机制。方法采用实时定量聚合酶链反应检测PGM5-AS1和miR-18a-3p水平。通过Kaplan-Meier生存分析和多变量Cox回归分析评估预后意义。利用细胞计数试剂盒-8测定乳腺癌细胞的细胞活力。用transwell法观察细胞迁移和侵袭。通过双荧光素酶报告基因测定验证PGM5-AS1、miR-18a-3p和TGFBR3之间的靶向调控相互作用。结果PGM5-AS1在乳腺组织和肿瘤细胞系中表达水平均较低。这种表达的减少与乳腺癌患者的各种临床特征和总体存活率的降低有关。PGM5-AS1表达上调可显著抑制乳腺癌细胞的增殖、迁移和侵袭。PGM5-AS1通过控制miR-18a-3p/TGFBR3轴调控乳腺癌的发展。结论lncRNA PGM5-AS1/miR-18a-3p/TGFBR3轴被认为是乳腺癌治疗发展的潜在遗传靶点。

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来源期刊

International Journal of Biological Markers 医学-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

期刊介绍： IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.