Analysis of gene expression within individual cells reveals spatiotemporal patterns underlying Vibrio cholerae biofilm development.

IF 9.8 1区 生物学 Q1 Agricultural and Biological Sciences
PLoS Biology Pub Date : 2025-05-16 eCollection Date: 2025-05-01 DOI:10.1371/journal.pbio.3003187
Grace E Johnson, Chenyi Fei, Ned S Wingreen, Bonnie L Bassler
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引用次数: 0

Abstract

Bacteria commonly exist in multicellular, surface-attached communities called biofilms. Biofilms are central to ecology, medicine, and industry. The Vibrio cholerae pathogen forms biofilms from single founder cells that, via cell division, mature into three-dimensional structures with distinct, yet reproducible, regional architectures. To define mechanisms underlying biofilm developmental transitions, we establish a single-molecule fluorescence in situ hybridization (smFISH) approach that enables accurate quantitation of spatiotemporal gene-expression patterns in biofilms at cell-scale resolution. smFISH analyses of V. cholerae biofilm regulatory and structural genes demonstrate that, as biofilms mature, overall matrix gene expression decreases, and simultaneously, a pattern emerges in which matrix gene expression becomes largely confined to peripheral biofilm cells. Both quorum sensing and c-di-GMP-signaling are required to generate the proper temporal pattern of matrix gene expression. Quorum sensing signaling is uniform across the biofilm, and thus, c-di-GMP-signaling alone sets the regional matrix gene expression pattern. The smFISH strategy provides insight into mechanisms conferring particular fates to individual biofilm cells.

单个细胞内基因表达的分析揭示了霍乱弧菌生物膜发育的时空模式。
细菌通常存在于称为生物膜的多细胞表面附着群落中。生物膜是生态学、医学和工业的核心。霍乱弧菌病原体从单个创始细胞形成生物膜,通过细胞分裂成熟为具有独特但可复制的区域结构的三维结构。为了确定生物膜发育转变的机制,我们建立了一种单分子荧光原位杂交(smFISH)方法,可以在细胞尺度分辨率下精确定量生物膜中的时空基因表达模式。smFISH对霍乱弧菌生物膜调控基因和结构基因的分析表明,随着生物膜的成熟,整体基质基因表达减少,同时出现基质基因表达主要局限于外周生物膜细胞的模式。群体感应和c-di- gmp信号都需要产生适当的基质基因表达的时间模式。群体感应信号在整个生物膜上是均匀的,因此,c-di- gmp信号单独设置了区域基质基因表达模式。smFISH策略提供了对赋予单个生物膜细胞特定命运的机制的深入了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PLoS Biology
PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY
CiteScore
15.40
自引率
2.00%
发文量
359
审稿时长
3-8 weeks
期刊介绍: PLOS Biology is the flagship journal of the Public Library of Science (PLOS) and focuses on publishing groundbreaking and relevant research in all areas of biological science. The journal features works at various scales, ranging from molecules to ecosystems, and also encourages interdisciplinary studies. PLOS Biology publishes articles that demonstrate exceptional significance, originality, and relevance, with a high standard of scientific rigor in methodology, reporting, and conclusions. The journal aims to advance science and serve the research community by transforming research communication to align with the research process. It offers evolving article types and policies that empower authors to share the complete story behind their scientific findings with a diverse global audience of researchers, educators, policymakers, patient advocacy groups, and the general public. PLOS Biology, along with other PLOS journals, is widely indexed by major services such as Crossref, Dimensions, DOAJ, Google Scholar, PubMed, PubMed Central, Scopus, and Web of Science. Additionally, PLOS Biology is indexed by various other services including AGRICOLA, Biological Abstracts, BIOSYS Previews, CABI CAB Abstracts, CABI Global Health, CAPES, CAS, CNKI, Embase, Journal Guide, MEDLINE, and Zoological Record, ensuring that the research content is easily accessible and discoverable by a wide range of audiences.
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