Associations between MTA1 and the outcome of oral leukoplakia: evidence from cohort studies and functional analyses.

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2025-04-21 DOI:10.1016/j.oooo.2025.04.005

Qiyue Zhang, Lin Ye, Ying Wang, Tiantian Xu, Shiyu Zhang, Zhiyong Wang, Qianming Chen, Jiang Liu, Xin Zeng, Jing Li

{"title":"Associations between MTA1 and the outcome of oral leukoplakia: evidence from cohort studies and functional analyses.","authors":"Qiyue Zhang, Lin Ye, Ying Wang, Tiantian Xu, Shiyu Zhang, Zhiyong Wang, Qianming Chen, Jiang Liu, Xin Zeng, Jing Li","doi":"10.1016/j.oooo.2025.04.005","DOIUrl":null,"url":null,"abstract":"Objective: To describe the relationship between MTA1 expression and Oral leukoplakia (OLK) prognosis in cohort studies and its possible mechanism.Materials and methods: We assessed MTA1 expression levels in OLK and OSCC tissues and cell lines using immunohistochemistry and Western blot. Functional analyses were performed in vitro by CCK-8 and Western blot assays. We analyzed overall survival in our OSCC cohort and TCGA dataset and analyzed the microenvironmental landscape of different MTA1 expression patterns in OLK and OSCC by multiplex immunohistochemistry. The Cox proportional hazards regression model was used to determine multivariate hazard ratios for overall survival.Results: MTA1 is abnormally highly expressed in OLK and OSCC both in cells and tissue, promoting DOK cell proliferation and altering EMT through E-cadherin, Wnt3a, and β-catenin. Moreover, MTA1 gradually increased with increasing abnormal OLK proliferation and negatively correlated with OSCC prognosis. Notably, MTA1 shows a positive correlation with M2 macrophage infiltration levels. Additionally, we found that low expression of MTA1 and a low M2/M1 ratio panel predict good patient prognosis in OSCC.Conclusion: We suggest that the aberrant expression of MTA1 may contribute to the malignant transformation of OLK and that MTA1 may represent a new prognostic marker for OLK and OSCC.","PeriodicalId":49010,"journal":{"name":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","volume":" ","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral Surgery Oral Medicine Oral Pathology Oral Radiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.oooo.2025.04.005","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To describe the relationship between MTA1 expression and Oral leukoplakia (OLK) prognosis in cohort studies and its possible mechanism.

Materials and methods: We assessed MTA1 expression levels in OLK and OSCC tissues and cell lines using immunohistochemistry and Western blot. Functional analyses were performed in vitro by CCK-8 and Western blot assays. We analyzed overall survival in our OSCC cohort and TCGA dataset and analyzed the microenvironmental landscape of different MTA1 expression patterns in OLK and OSCC by multiplex immunohistochemistry. The Cox proportional hazards regression model was used to determine multivariate hazard ratios for overall survival.

Results: MTA1 is abnormally highly expressed in OLK and OSCC both in cells and tissue, promoting DOK cell proliferation and altering EMT through E-cadherin, Wnt3a, and β-catenin. Moreover, MTA1 gradually increased with increasing abnormal OLK proliferation and negatively correlated with OSCC prognosis. Notably, MTA1 shows a positive correlation with M2 macrophage infiltration levels. Additionally, we found that low expression of MTA1 and a low M2/M1 ratio panel predict good patient prognosis in OSCC.

Conclusion: We suggest that the aberrant expression of MTA1 may contribute to the malignant transformation of OLK and that MTA1 may represent a new prognostic marker for OLK and OSCC.

查看原文本刊更多论文

MTA1与口腔白斑结局之间的关系：来自队列研究和功能分析的证据。

目的：探讨队列研究中MTA1表达与口腔白斑（OLK）预后的关系及其可能的机制。材料和方法：我们使用免疫组织化学和Western blot方法评估了MTA1在OLK和OSCC组织和细胞系中的表达水平。体外用CCK-8和Western blot进行功能分析。我们分析了我们的OSCC队列和TCGA数据集的总生存率，并通过多重免疫组织化学分析了OLK和OSCC中不同MTA1表达模式的微环境景观。Cox比例风险回归模型用于确定总生存率的多变量风险比。结果：MTA1在细胞和组织中均在OLK和OSCC中异常高表达，通过E-cadherin、Wnt3a和β-catenin促进DOK细胞增殖，改变EMT。MTA1随着异常OLK增殖的增加而逐渐升高，与OSCC预后呈负相关。值得注意的是，MTA1与M2巨噬细胞浸润水平呈正相关。此外，我们发现MTA1的低表达和M2/M1的低比值预示着OSCC患者预后良好。结论：MTA1的异常表达可能与OLK的恶性转化有关，MTA1可能是OLK和OSCC的一个新的预后指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.