Casein phosphopeptide-amorphous- calcium phosphate's effect on enamel microhardness of teeth treated with nano silver in sodium fluoride solution.

Abstract

Objective: Aim: To detect the effect of an experimental 0.7% nanosilver in sodium fluoride (NSSF) and compare it to that of 5% sodium fluoride (NaF) on demineralized teeth, also observes the impact of applying 10% Casein phosphopeptide-amorphous-calcium phosphate (CPP-ACP) on those groups and compares that to CPP-ACP's lone impact on demineralized teeth.

Patients and methods: Materials and Methods: The sample consisted of 60 sound, premolar teeth without hypo-mineralization or cracks. They were divided into three groups following the formation of caries-like lesions using a Feather Stone pH cycle. Group No.1 was treated with NSSF, group No.2 was treated with NaF, and deionized water was used for Group No.3. All groups had two minutes of application time and were then stored for 24 hours in artificial saliva. After that, they were treated with CPP-ACP, for seven days twice a day. Microhardness measurements were carried out four times: before any intervention, after the pH cycle, after 24 hours from the application of NSSF and NaF, and a final one after seven days from the application of CPP-ACP on the previous groups. An XRF, XRD analysis, and particle size analyzer were used to confirm the nanosilver properties of the powder.

Results: Results: There was a statistically significant increase in microhardness values (P<0.05) following CPP-ACP's application on teeth previously subjected to NSSF and NaF. The group that was subjected to deionized water before CPP-ACP's application revealed a statistically significant value (P<0.001).

Conclusion: Conclusions: CPP-ACP enhances the microhardness and, hence, the mineralization of teeth previously treated with NSSF and NaF solutions.