Optimization of sustainable bioprocessing for the production of high-value biomolecules (chlorophylls and proteins) using Desmodesmus subspicatus.

Abstract

Traditional protein and pigment (e.g., chlorophyll) sources are becoming insufficient due to the rapid rise of the global population in modern civilization. Microalgae offer a promising solution for protein and chlorophyll sources due to their higher productivity than terrestrial plants. This study aims to optimize the cultivation conditions for Desmodesmus subspicatus, a microalgal strain containing ∼60% protein and 4% chlorophyll, to enhance biomass, protein and chlorophyll productivity. A Taguchi Orthogonal Array (TOA) was used for systematic optimization of BG-11 medium components. Further experiments assessed the effects of light intensity and different carbon and nitrogen sources. Under optimized BG-11 conditions, biomass increased 1.3-fold, with protein and chlorophyll productivity rising 2.25 and 1.92-fold, respectively. Supplementation with carbon and nitrogen sources under varying light (84-504 µmol m^-2 s^-1) further enhanced yields by 1.6-fold. Glycine proved to be the most effective nitrogen source, while cellulose as a carbon source resulted in 2.4-fold higher biomass, 7.3-fold higher protein, and 2.3-fold higher chlorophyll. Cytotoxicity assessment of the extracted chlorophyll revealed over 94% A549 cell viability at concentrations up to 100 µg/mL, confirming its biocompatibility. Therefore, Desmodesmus subspicatus has promise as a sustainable source of proteins and chlorophylls in the nutraceutical and food industries.