Phosphate concentrations in follicular fluid during assisted reproductive treatment: relevance for ovarian function and fertility outcomes.

IF 4.7 2区医学 Q1 ENDOCRINOLOGY & METABOLISM

Reproductive Biology and Endocrinology Pub Date : 2025-05-14 DOI:10.1186/s12958-025-01408-w

Signe Marie Wulff, Mads Joon Jorsal, Ireen Kooij, Hans Krog, Ursula Bentin-Ley, Martin Blomberg Jensen, Sam Kafai Yahyavi

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Abstract

Background: The role of follicular fluid phosphate for reproductive health and oocyte maturation is unclear. This study investigates the relationship between follicular fluid vs serum phosphate concentrations and the possible link with sex steroids during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) at a Danish fertility clinic.

Methods: A prospective cohort of infertile women who attended Danfert Fertility clinic (Copenhagen, Denmark) and received IVF or ICSI treatment between June 2015 and February 2017. Correlation analyses were performed with Spearman's Rank or Pearson's correlation, while categorical variables were analyzed with Chi-squared test.

Results: In total, 110 participants were included in the study, and 33 of these achieved a live birth. Phosphate concentrations were higher in the follicular fluid compared to corresponding serum samples (1.16 mmol/L vs. 1.06 mmol/L, p = 0.002) and there was a positive correlation between serum and follicular fluid phosphate concentrations (r = 0.43, p = 0.007). A positive trend was also found for calcium concentrations, though not statistically significant (r = 0.31, p = 0.060). Correlation analysis also showed a positive correlation between concentrations of phosphate and calcium in follicular fluid (r = 0.41, p < 0.001). A positive correlation was observed between concentrations of phosphate and testosterone in follicular fluid (r = 0.34, p < 0.001). When stratified into tertiles, we found no significant differences between live birth rates in follicular fluid phosphate (p = 0.624), calcium (p = 0.207), or testosterone (p = 0.841).

Conclusions: This study found that follicular fluid phosphate concentrations are higher than serum phosphate concentrations, suggesting possible local regulation. However, no significant association was found between follicular phosphate and ART outcomes. Further research is needed to explore its potential role in reproductive physiology.

Clinical trial: Clinicaltrials.gov (NCT02437578; registration date 2015/04/16).

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辅助生殖治疗期间卵泡液中的磷酸盐浓度：与卵巢功能和生育结果的相关性

背景：卵泡液磷酸盐在生殖健康和卵母细胞成熟中的作用尚不清楚。本研究调查了丹麦一家生育诊所在体外受精（IVF）或胞浆内单精子注射（ICSI）期间卵泡液与血清磷酸盐浓度之间的关系以及与性类固醇的可能联系。方法：2015年6月至2017年2月期间，在丹麦哥本哈根的Danfert Fertility clinic就诊并接受IVF或ICSI治疗的不孕女性的前瞻性队列。相关分析采用Spearman’s Rank或Pearson相关，分类变量分析采用卡方检验。结果：总共有110名参与者被纳入研究，其中33名实现了活产。卵泡液中磷酸盐浓度高于相应的血清样本（1.16 mmol/L vs. 1.06 mmol/L, p = 0.002），血清和卵泡液中磷酸盐浓度呈正相关（r = 0.43, p = 0.007）。钙浓度也呈阳性趋势，但无统计学意义（r = 0.31, p = 0.060）。相关分析还显示卵泡液中磷酸盐和钙的浓度呈正相关（r = 0.41, p）。结论：本研究发现卵泡液中磷酸盐浓度高于血清中磷酸盐浓度，提示可能存在局部调节。然而，卵泡磷酸盐与ART结果之间没有发现显著关联。其在生殖生理中的潜在作用有待进一步研究。临床试验：Clinicaltrials.gov (NCT02437578；注册日期2015年4月16日)。

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来源期刊

Reproductive Biology and Endocrinology 医学-内分泌学与代谢

CiteScore

7.90

自引率

2.30%

发文量

161

审稿时长

4-8 weeks

期刊介绍： Reproductive Biology and Endocrinology publishes and disseminates high-quality results from excellent research in the reproductive sciences. The journal publishes on topics covering gametogenesis, fertilization, early embryonic development, embryo-uterus interaction, reproductive development, pregnancy, uterine biology, endocrinology of reproduction, control of reproduction, reproductive immunology, neuroendocrinology, and veterinary and human reproductive medicine, including all vertebrate species.