RNA-Sequencing in Elucidating Immune Responses to Haemonchus contortus Infection in Small Ruminants: Systematic Review.

IF 1.4 4区 医学 Q4 IMMUNOLOGY
Bongeka Sylvia Ndaba, Erika Faber, Munyaradzi Christopher Marufu, Alri Pretorius, Selaelo Ivy Tshilwane
{"title":"RNA-Sequencing in Elucidating Immune Responses to Haemonchus contortus Infection in Small Ruminants: Systematic Review.","authors":"Bongeka Sylvia Ndaba, Erika Faber, Munyaradzi Christopher Marufu, Alri Pretorius, Selaelo Ivy Tshilwane","doi":"10.1111/pim.70009","DOIUrl":null,"url":null,"abstract":"<p><p>Haemonchus contortus poses a major threat to small ruminant production in subtropical regions worldwide. Unfortunately, there is growing anthelmintic resistance, and the only licensed vaccine has limitations. This paper aimsto review the use of RNA-sequencing in understanding the immune responses of small ruminants to H. contortus infection, focusing on identifying differentially expressed genes and elucidating key immune pathways associated with resistance and susceptibility. This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement guidelines and Population, Intervention, Control, and Outcome framework covering publications from January 2015 to December 2023. RNA-seq identified the activation of key immune pathways, such as Th1/Th2, NK cell, B cell receptor signalling, MAPK, CAMs, and TNF signalling. There was upregulation of a range of PRRs, including TLRs and CLECs, in the resistant sheep, suggesting a crucial role for trained innate immune cells in resistance. However, there are no direct comparisons of TLR and CLEC expression between resistant and susceptible goats. This shows that there is a gap in understanding of the immune response mechanisms in goats. Addressing these knowledge gaps will lead to the development of more effective and sustainable control strategies.</p>","PeriodicalId":19931,"journal":{"name":"Parasite Immunology","volume":"47 5","pages":"e70009"},"PeriodicalIF":1.4000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079082/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasite Immunology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/pim.70009","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Haemonchus contortus poses a major threat to small ruminant production in subtropical regions worldwide. Unfortunately, there is growing anthelmintic resistance, and the only licensed vaccine has limitations. This paper aimsto review the use of RNA-sequencing in understanding the immune responses of small ruminants to H. contortus infection, focusing on identifying differentially expressed genes and elucidating key immune pathways associated with resistance and susceptibility. This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement guidelines and Population, Intervention, Control, and Outcome framework covering publications from January 2015 to December 2023. RNA-seq identified the activation of key immune pathways, such as Th1/Th2, NK cell, B cell receptor signalling, MAPK, CAMs, and TNF signalling. There was upregulation of a range of PRRs, including TLRs and CLECs, in the resistant sheep, suggesting a crucial role for trained innate immune cells in resistance. However, there are no direct comparisons of TLR and CLEC expression between resistant and susceptible goats. This shows that there is a gap in understanding of the immune response mechanisms in goats. Addressing these knowledge gaps will lead to the development of more effective and sustainable control strategies.

rna测序在阐明小反刍动物对弯曲血蜱感染的免疫反应中的应用:系统综述。
弯血蜱是全球亚热带地区小反刍动物生产的主要威胁。不幸的是,驱虫病的抗药性越来越强,而唯一获得许可的疫苗也有局限性。本文旨在综述rna测序在了解小反刍动物对H. contortus感染的免疫反应方面的应用,重点是鉴定差异表达基因和阐明与耐药和易感性相关的关键免疫途径。本综述遵循系统评价和荟萃分析声明指南的首选报告项目和人口、干预、控制和结果框架,涵盖2015年1月至2023年12月的出版物。RNA-seq鉴定了关键免疫通路的激活,如Th1/Th2、NK细胞、B细胞受体信号、MAPK、CAMs和TNF信号。在耐药羊中,一系列PRRs(包括tlr和clc)上调,这表明训练有素的先天免疫细胞在耐药中起着至关重要的作用。然而,在耐药山羊和易感山羊之间没有TLR和CLEC表达的直接比较。这表明对山羊免疫反应机制的理解存在差距。解决这些知识差距将导致制定更有效和可持续的控制战略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Parasite Immunology
Parasite Immunology 医学-寄生虫学
CiteScore
4.70
自引率
4.50%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Parasite Immunology is an international journal devoted to research on all aspects of parasite immunology in human and animal hosts. Emphasis has been placed on how hosts control parasites, and the immunopathological reactions which take place in the course of parasitic infections. The Journal welcomes original work on all parasites, particularly human parasitology, helminths, protozoa and ectoparasites.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信