Protective effect of safflower oil supplementation on sciatic nerve crush injury in rats.

IF 3.6 4区医学 Q2 NEUROSCIENCES

Nutritional Neuroscience Pub Date : 2025-10-01 Epub Date: 2025-05-16 DOI:10.1080/1028415X.2025.2506461

Qin Li, Yuting Wang, Songyi Wei, Juan Liu, Jun Wang

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引用次数: 0

Abstract

Objective: This study aimed to assess the effect of safflower oil (SO), a high-quality edible oil derived from seeds of Carthamus tinctorius L. possessing multiple bioactivities, on rats that underwent sciatic nerve crush (SNC) to induce peripheral nerve injury (PNI).

Methods: Sprague-Dawley rats were randomly divided into 4 groups with 10 mice in each group: sham, SNC model, SNC + SO 100 mg/kg, and SNC + SO 300 mg/kg. The sciatic function index (SFI) was monitored for evaluation of sciatic nerve function. Bilateral gastrocnemius muscles were isolated to measure the gastrocnemius muscle mass ratio. Tissue sections of sciatic nerve and gastrocnemius muscle were stained with hematoxylin and eosin for histopathological observations. The levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, malondialdehyde (MDA), and superoxide dismutase (SOD), and expression of myelin basic protein(MBP) and β₃-tubulin were detected in damaged nerves.

Results: SO supplementation increased the SFI scores, improved the histopathological features of damaged nerves, and enhanced muscle mass and fiber diameters of affected gastrocnemius muscle in SNC rats. SNC-enhanced levels of TNF-α, IL-1β and MDA in damaged nerves were reduced, while SNC-inhibited SOD activity was increased by SO supplementation. Nerve expression of MBP and β₃-tubulin was upregulated in SO-supplemented SNC rats.

Conclusion: Date suggested that SO could improve nerve function, attenuate neuroinflammation and oxidative stress, and might accelerate peripheral nerve regeneration after injury in SNC rats, thus being a promising dietary adjuvant for PNI treatment.

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红花油对大鼠坐骨神经挤压损伤的保护作用。

目的：研究红花油对坐骨神经压迫（SNC）大鼠周围神经损伤（PNI）的保护作用。红花油是一种从红花籽中提取的具有多种生物活性的优质食用油。方法：将Sprague-Dawley大鼠随机分为4组，每组10只：假手术组、SNC模型组、SNC + SO 100 mg/kg组、SNC + SO 300 mg/kg组。监测坐骨神经功能指数（SFI）评价坐骨神经功能。分离双侧腓肠肌，测量腓肠肌质量比。用苏木精和伊红染色观察坐骨神经和腓肠肌组织切片的病理变化。检测损伤神经组织中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、丙二醛（MDA）、超氧化物歧化酶（SOD）水平及髓鞘碱性蛋白（MBP）、β3-微管蛋白的表达。结果：补充SO可提高SNC大鼠SFI评分，改善受损神经的组织病理学特征，增加患腓肠肌的肌肉质量和纤维直径。添加SO后，受损神经中snc增强的TNF-α、IL-1β和MDA水平降低，snc抑制的SOD活性升高。SNC大鼠神经中MBP和β3-微管蛋白的表达上调。结论：实验结果表明，SO可改善SNC大鼠损伤后神经功能，减轻神经炎症和氧化应激，并可能促进周围神经再生，是治疗PNI的一种有前景的膳食佐剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nutritional Neuroscience 医学-神经科学

CiteScore

8.50

自引率

2.80%

发文量

236

审稿时长

>12 weeks

期刊介绍： Nutritional Neuroscience is an international, interdisciplinary broad-based, online journal for reporting both basic and clinical research in the field of nutrition that relates to the central and peripheral nervous system. Studies may include the role of different components of normal diet (protein, carbohydrate, fat, moderate use of alcohol, etc.), dietary supplements (minerals, vitamins, hormones, herbs, etc.), and food additives (artificial flavours, colours, sweeteners, etc.) on neurochemistry, neurobiology, and behavioural biology of all vertebrate and invertebrate organisms. Ideally this journal will serve as a forum for neuroscientists, nutritionists, neurologists, psychiatrists, and those interested in preventive medicine.