Plasmopara viticola Effector PvRXLR10 Targets a Host Phospholipase VvipPLA-IIδ2 to Suppress Plant Immunity in Grapevine.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Junjie Qu, Lulu Liu, Chengxu Zheng, Zexi Guo, Dayun Sun, Fengying Pan, Jiang Lu, Ling Yin
{"title":"Plasmopara viticola Effector PvRXLR10 Targets a Host Phospholipase VvipPLA-IIδ2 to Suppress Plant Immunity in Grapevine.","authors":"Junjie Qu, Lulu Liu, Chengxu Zheng, Zexi Guo, Dayun Sun, Fengying Pan, Jiang Lu, Ling Yin","doi":"10.1111/mpp.70095","DOIUrl":null,"url":null,"abstract":"<p><p>Plasmopara viticola that causes grapevine downy mildew disease in viticulture regions is among the 10 most relevant pathogens worldwide. It secretes a large arsenal of effectors to facilitate colonisation by perturbing host immunity. However, the underlying mechanisms by which P. viticola effectors disturb grapevine defence are still largely unknown. In this study, we report that PvRXLR10, an RXLR effector with a WY domain, promotes P. viticola infection in grapevine and Phytophthora parasitica colonisation in Nicotiana benthamiana. PvRXLR10 interacts with a host patatin-like protein VvipPLA-IIδ2 with phospholipase A2 activity. The WY domain of PvRXLR10 is not responsible for cell death suppression in N. benthamiana but is necessary for PvRXLR10 interaction with VvipPLA-IIδ2. Overexpression and RNAi-mediated suppression of VvipPLA-IIδ2 expression in Vitis vinifera consistently showed that this protein positively regulates plant immunity in response to P. viticola infection. Interestingly, we found that VvipPLA-IIδ2 partially associates with PvRXLR10 at the endoplasmic reticulum (ER). Reverse transcription-quantitative PCR (RT-qPCR) analysis showed that the expression of VvipPLA-IIδ2 was suppressed by PvRXLR10 during P. viticola infection. The overexpression of VvipPLA-IIδ2 in V. vinifera induced higher expression of genes related to jasmonic acid (JA) biosynthesis, signalling pathways and defence response. The evidence indicates the important roles of VvipPLA-IIδ2 in grapevine immunity and P. viticola effector PvRXLR10 targets this protein to promote its infection.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70095"},"PeriodicalIF":4.8000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081833/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.70095","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Plasmopara viticola that causes grapevine downy mildew disease in viticulture regions is among the 10 most relevant pathogens worldwide. It secretes a large arsenal of effectors to facilitate colonisation by perturbing host immunity. However, the underlying mechanisms by which P. viticola effectors disturb grapevine defence are still largely unknown. In this study, we report that PvRXLR10, an RXLR effector with a WY domain, promotes P. viticola infection in grapevine and Phytophthora parasitica colonisation in Nicotiana benthamiana. PvRXLR10 interacts with a host patatin-like protein VvipPLA-IIδ2 with phospholipase A2 activity. The WY domain of PvRXLR10 is not responsible for cell death suppression in N. benthamiana but is necessary for PvRXLR10 interaction with VvipPLA-IIδ2. Overexpression and RNAi-mediated suppression of VvipPLA-IIδ2 expression in Vitis vinifera consistently showed that this protein positively regulates plant immunity in response to P. viticola infection. Interestingly, we found that VvipPLA-IIδ2 partially associates with PvRXLR10 at the endoplasmic reticulum (ER). Reverse transcription-quantitative PCR (RT-qPCR) analysis showed that the expression of VvipPLA-IIδ2 was suppressed by PvRXLR10 during P. viticola infection. The overexpression of VvipPLA-IIδ2 in V. vinifera induced higher expression of genes related to jasmonic acid (JA) biosynthesis, signalling pathways and defence response. The evidence indicates the important roles of VvipPLA-IIδ2 in grapevine immunity and P. viticola effector PvRXLR10 targets this protein to promote its infection.

葡萄原浆原效应物PvRXLR10靶向宿主磷脂酶VvipPLA-IIδ2抑制葡萄植物免疫
在葡萄种植区引起葡萄霜霉病的葡萄浆原菌是世界上10种最相关的病原体之一。它分泌大量的效应物,通过扰乱宿主免疫来促进定植。然而,葡萄球菌效应物干扰葡萄藤防御的潜在机制在很大程度上仍然未知。在本研究中,我们报道了一个带有WY结构域的RXLR效应物PvRXLR10,它能促进葡萄病原菌感染和本烟霉疫霉菌的定殖。PvRXLR10与宿主patatin样蛋白vvippla - i δ2相互作用,具有磷脂酶A2活性。PvRXLR10的WY结构域与benthamiana细胞死亡抑制无关,但对于PvRXLR10与vvippla - i δ2的相互作用是必需的。vvippla - i- δ2蛋白在葡萄中的过表达和rnai介导的抑制一致表明该蛋白在葡萄侵染过程中积极调节植物免疫应答。有趣的是,我们发现vvippla - i δ2在内质网(ER)与PvRXLR10部分结合。逆转录-定量PCR (RT-qPCR)分析显示,PvRXLR10在葡萄假单胞菌感染过程中抑制了vvippla - i - δ2的表达。vvippla - i δ2在葡萄花中的过表达诱导茉莉酸(jasmonic acid, JA)生物合成、信号通路和防御反应相关基因的高表达。证据表明,vvippla - i - δ2在葡萄免疫中起重要作用,葡萄假单胞菌效应物PvRXLR10靶向该蛋白促进其感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信