{"title":"Canonical Wnt Pathway Enhanced Dental Pulp Mesenchymal Stem Cells-Mediated Cementum Regeneration.","authors":"Dongmei Cheng, Xin Yuan, Zhiyu Ma, Xiaoyu Lin, Zhao Zhu, Jinsong Wang, Chunmei Zhang, Yuji Wang, Jian Zhou, Songlin Wang, Lei Hu","doi":"10.1111/joor.14001","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Cementum is a mineralised tissue that is essential for maintaining periodontal tissue homeostasis. Our previous research has shown that dental pulp mesenchymal stem cells (DPSCs) can regenerate periodontal tissues. However, the regeneration efficiency and the specific molecular mechanism remain unclear.</p><p><strong>Objective: </strong>This study aimed to clarify the expression differences of the Wnt signalling pathway in cementum, dental pulp and periodontal ligament and illustrate the function of Wnt3a in promoting cementum regeneration of DPSCs.</p><p><strong>Methods: </strong>RNA Smart transcriptome sequencing was performed to illustrate the differential gene expression of the Wnt pathway among cementum, dental pulp and periodontal tissues. Biological function experiments of Wnt3a were conducted to demonstrate the capacity of Wnt3a to enhance cementum regeneration of DPSCs. The specific mechanism of Wnt3a function was clarified through single-cell sequencing and western blotting.</p><p><strong>Results: </strong>The expression of the canonical Wnt signalling pathway, especially Wnt3a, was significantly higher in cementum, while DPSCs mainly express the non-canonical Wnt signalling pathway. Furthermore, Wnt3a could enhance the cementum regeneration of DPSCs in vitro and in vivo. Single-cell RNA-seq analysis showed that Wnt3a promoted the cementogenic differentiation of DPSCs via the classical Wnt signalling pathway.</p><p><strong>Conclusions: </strong>The results unveiled that the non-classical Wnt signalling pathway of DPSCs would limit the efficiency of cementum regeneration. Wnt3a could improve the efficacy of DPSCs-mediated cementum regeneration through activating the canonical Wnt pathway, which may offer a promising therapeutic approach to tooth regeneration.</p>","PeriodicalId":16605,"journal":{"name":"Journal of oral rehabilitation","volume":" ","pages":""},"PeriodicalIF":3.1000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral rehabilitation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/joor.14001","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Cementum is a mineralised tissue that is essential for maintaining periodontal tissue homeostasis. Our previous research has shown that dental pulp mesenchymal stem cells (DPSCs) can regenerate periodontal tissues. However, the regeneration efficiency and the specific molecular mechanism remain unclear.
Objective: This study aimed to clarify the expression differences of the Wnt signalling pathway in cementum, dental pulp and periodontal ligament and illustrate the function of Wnt3a in promoting cementum regeneration of DPSCs.
Methods: RNA Smart transcriptome sequencing was performed to illustrate the differential gene expression of the Wnt pathway among cementum, dental pulp and periodontal tissues. Biological function experiments of Wnt3a were conducted to demonstrate the capacity of Wnt3a to enhance cementum regeneration of DPSCs. The specific mechanism of Wnt3a function was clarified through single-cell sequencing and western blotting.
Results: The expression of the canonical Wnt signalling pathway, especially Wnt3a, was significantly higher in cementum, while DPSCs mainly express the non-canonical Wnt signalling pathway. Furthermore, Wnt3a could enhance the cementum regeneration of DPSCs in vitro and in vivo. Single-cell RNA-seq analysis showed that Wnt3a promoted the cementogenic differentiation of DPSCs via the classical Wnt signalling pathway.
Conclusions: The results unveiled that the non-classical Wnt signalling pathway of DPSCs would limit the efficiency of cementum regeneration. Wnt3a could improve the efficacy of DPSCs-mediated cementum regeneration through activating the canonical Wnt pathway, which may offer a promising therapeutic approach to tooth regeneration.
期刊介绍：
Journal of Oral Rehabilitation aims to be the most prestigious journal of dental research within all aspects of oral rehabilitation and applied oral physiology. It covers all diagnostic and clinical management aspects necessary to re-establish a subjective and objective harmonious oral function.
Oral rehabilitation may become necessary as a result of developmental or acquired disturbances in the orofacial region, orofacial traumas, or a variety of dental and oral diseases (primarily dental caries and periodontal diseases) and orofacial pain conditions. As such, oral rehabilitation in the twenty-first century is a matter of skilful diagnosis and minimal, appropriate intervention, the nature of which is intimately linked to a profound knowledge of oral physiology, oral biology, and dental and oral pathology.
The scientific content of the journal therefore strives to reflect the best of evidence-based clinical dentistry. Modern clinical management should be based on solid scientific evidence gathered about diagnostic procedures and the properties and efficacy of the chosen intervention (e.g. material science, biological, toxicological, pharmacological or psychological aspects). The content of the journal also reflects documentation of the possible side-effects of rehabilitation, and includes prognostic perspectives of the treatment modalities chosen.
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