MiR-223-3p promotes angiogenesis in burn wound healing by targeting FOXO1.

Abstract

Deep second-degree burns represent a prevalent form of trauma encountered in clinical settings. The intricacies of the wound healing process are profoundly linked to the prognosis of affected patients. This study aims to explore the expression of miR-223-3p in deep second-degree burns and its role in wound healing. A total of 95 patients with deep second-degree burn were enrolled in this study and 50 healthy individuals were included in this study. Real-time quantitative PCR was employed to assess the expression levels of miR-223-3p. The TargetScan database was utilized to predict the target genes of miR-223-3p, and the luciferase reporter gene assay was employed to validate the predicted results. Pearson correlation analysis was conducted to examine the connection. The results indicate that the level of miR-223-3p was markedly increased in patients with deep second-degree burns and markedly decreased on the 28th day of wound healing. FOXO1 was the target gene of miR-223-3p. The level of miR-223-3p at the time of burn and after recovery is significantly negatively related to the expression of FOXO1 in patients with deep second-degree burn. Overexpression of miR-223-3p significantly inhibits the viability of HUVECs, while FOXO1 can partially reverse this inhibitory effect. In conclusion, the level of miR-223-3p is associated with the progression of deep second-degree burns, and it may participate in the wound healing process of burn injuries by targeting FOXO1.