Synthesis and Antibacterial Studies of Silver Loaded TiO₂ Electrospun Nanofibers Against E. coli by Fluorescence Spectroscopy and Microscopy.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2025-05-15 DOI:10.1007/s10895-025-04332-9

Bisma Khanam, Rafaqat Ali Khan, Shahzad Anwar, Hina Ali, Muhammad Zakria, Taj Muhammad Khan, A Rahman, Ahmad Adnan, Nida Ali Shan, Muhammad Raffi

{"title":"Synthesis and Antibacterial Studies of Silver Loaded TiO2 Electrospun Nanofibers Against E. coli by Fluorescence Spectroscopy and Microscopy.","authors":"Bisma Khanam, Rafaqat Ali Khan, Shahzad Anwar, Hina Ali, Muhammad Zakria, Taj Muhammad Khan, A Rahman, Ahmad Adnan, Nida Ali Shan, Muhammad Raffi","doi":"10.1007/s10895-025-04332-9","DOIUrl":null,"url":null,"abstract":"In healthcare, bacterial infections always remain a critical challenge. In this study, pristine and Ag-TiO2 nanofibers (NFs) with varying silver (Ag) concentration (2wt.%, 4wt.%, 6wt.% and 8wt.%) were prepared by electrospinning, followed by calcination at 500 °C for 3 h. Morphological characterization revealed the formation of one-dimensional NFs with mean diameter 83.06 to 25.80 nm, depending on the Ag loading. The antibacterial activities were investigated using conventional methods (well diffusion, colony forming units counting) and advanced techniques (Confocal laser scanning fluorescence microscopy, Scanning electron microscopy and Fluorescence spectroscopy). The results from antibacterial studies have revealed that sample with 8wt.% Ag-TiO2 exhibited the most significant antibacterial effect as evidenced by marked reduction in CFUs, almost complete quenching of fluorescence emission intensity and perforated bacterial membranes and cellular debris.","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04332-9","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

In healthcare, bacterial infections always remain a critical challenge. In this study, pristine and Ag-TiO₂ nanofibers (NFs) with varying silver (Ag) concentration (2wt.%, 4wt.%, 6wt.% and 8wt.%) were prepared by electrospinning, followed by calcination at 500 °C for 3 h. Morphological characterization revealed the formation of one-dimensional NFs with mean diameter 83.06 to 25.80 nm, depending on the Ag loading. The antibacterial activities were investigated using conventional methods (well diffusion, colony forming units counting) and advanced techniques (Confocal laser scanning fluorescence microscopy, Scanning electron microscopy and Fluorescence spectroscopy). The results from antibacterial studies have revealed that sample with 8wt.% Ag-TiO₂ exhibited the most significant antibacterial effect as evidenced by marked reduction in CFUs, almost complete quenching of fluorescence emission intensity and perforated bacterial membranes and cellular debris.

查看原文本刊更多论文

载银二氧化钛静电纺丝纳米纤维的合成及其对大肠杆菌的抑菌研究

在医疗保健领域，细菌感染始终是一个严峻的挑战。在这项研究中，原始和Ag- tio2纳米纤维（NFs）具有不同的银（Ag）浓度（2wt）。wt %, 4。6 wt %。在500°C下煅烧3 h后，通过静电纺丝法制备了银纳米颗粒。形貌表征表明，银纳米颗粒的平均直径为83.06 ~ 25.80 nm。采用常规方法（孔扩散、菌落形成单位计数）和先进技术（共聚焦激光扫描荧光显微镜、扫描电镜和荧光光谱）对其抗菌活性进行了研究。抗菌研究结果表明，样品中含有8wt。Ag-TiO2表现出最显著的抗菌效果，可以明显降低cfu，几乎完全猝灭荧光发射强度，穿孔细菌膜和细胞碎片。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.