Biocontrol potential of bacteria associated with Asian walnut moth Erschoviella musculana Erschoff (Lepidoptera: Nolidae) on walnut pests.

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Microbiology Pub Date : 2025-05-16 DOI:10.1007/s10123-025-00674-3

Hande Bayraktar Şalvarci, Donus Gencer, Ardahan Eski, Vildan Bozkurt, İsmail Demir

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Abstract

The aim of this study was to investigate the culturable bacterial flora of Erschoviella musculana, which causes damage to leaves, shoots, branches, trunk, and fruits of walnut trees, in order to find a possible microbial control agent against walnut pests. In this study, a total of 11 bacteria were isolated from live and dead larvae of E. musculana and identified based on their morphological, biochemical, and molecular characteristics. Based on these results, the isolates were identified as Bacillus toyonensis EMB1, Bacillus toyonensis EMB3, Stenotrophomonas rhizophila EMO6, Stenotrophomonas rhizophila EM8, Delftia tsuruhatensis EM14, Acinetobacter modestus EM20, Lelliottia amnigena EM21, Bacillus toyonensis EM22, Pantoea vagans EM37, Pseudomonas alloputida EM42, and Stenotrophomonas geniculata EM43. The biological control potential of these isolates on E. musculana, Hyphantria cunea (Lepidoptera: Arctiidae), and Panaphis juglandis (Hemiptera: Callaphididae), which are very important walnut pests, was determined. The results showed that B. toyonensis strain EMB1 had the highest virulence (82%) on the larvae of H. cunea and S. rhizophila strain EM8 had the highest virulence (83%) on the nymphs of P. juglandis. On the other hand, S. geniculata strain EM43 caused 100% mortality on the larvae of E. musculana. The results of this study show that bacteria associated with E. musculana appear to be promising microbial control agents for walnut pests.

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亚洲核桃蛾相关细菌对核桃害虫的生物防效研究

摘要本研究旨在研究对核桃树叶、梢、枝、干和果实造成危害的肌肉Erschoviella musculana的可培养菌群，以期找到一种可能的核桃害虫微生物防治剂。本研究从活仔鱼和死仔鱼中分离出11种细菌，并对其形态、生化和分子特征进行了鉴定。结果表明，分离菌株为toyonensis EMB1、toyonensis EMB3、嗜根窄养单胞菌EMO6、嗜根窄养单胞菌EM8、tsuruhatdelftia EM14、modest不动杆菌EM20、水蛭杆菌EM21、toyonensis EM22、Pantoea vagans EM37、异臭假单胞菌EM42和genotrophomata EM43。测定了这些分离物对核桃重要害虫——肌肉田鼠、加利福尼亚棘球蛾（鳞翅目：针叶蛾科）和核桃木蚜（半翅目：callaphidae）的生物防治潜力。结果表明，toyonensis菌株EMB1对美洲圆蚧幼虫的毒力最高（82%），而S. rhizophilila菌株EM8对juglandis若虫的毒力最高（83%）。另一方面，霉菌菌株EM43对肌肉田鼠幼虫的死亡率为100%。本研究结果表明，与肌芽孢杆菌相关的细菌有望成为核桃害虫的微生物防治剂。

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来源期刊

International Microbiology 生物-生物工程与应用微生物

CiteScore

5.50

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials. A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.