An investigation of the therapeutic potential of the testicular tissue encapsulated in amnion membrane in mouse model: An experimental study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY

International Journal of Reproductive Biomedicine Pub Date : 2025-05-01 eCollection Date: 2024-05-01 DOI:10.18502/ijrm.v23i2.18489

Keykavoos Gholami, Elahe Asheghmadine, Fateme Guitynavard, Leila Zareian Baghdadabad, Diana Taheri, Parisa Zahmatkesh, Leonardo Oliveira Reis, Seyed Mohammad Kazem Aghamir

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Abstract

Background: Restoring fertility in male cancer individuals through testicular tissue transplantation faces challenges due to hypoxia-induced loss of spermatogonial stem cells (SSCs). Hydrogel encapsulation was explored to minimize hypoxic damage in testicular tissue transplantation. For this purpose, human amnion membrane (hAM)-derived hydrogel could be an alternative.

Objective: The potential of hAM-derived hydrogel to support testis tissue grafts was evaluated.

Materials and methods: In this experimental study, testicular tissue samples (1-3 mm³) were obtained from 16 male NMRI mice (4-5 wk, 22 $\pm$ 2 gr). These tissue fragments were either encapsulated within a hydrogel derived from a hAM or left unencapsulated (control) prior to being autologously transplanted beneath the dorsal skin of mice subjected to hemilateral or bilateral orchiectomy. The grafted testicular tissues were histologically evaluated for key parameters, including the integrity of seminiferous tubules, survival of SSCs, Sertoli cell functionality, as well as hypoxia and apoptosis on day 21.

Results: No significant differences were observed between groups regarding ST integrity, number of SSCs, Sertoli cell functionality, or the rate of hypoxia-inducible factor 1-alpha and apoptosis (p $\leq$ 0.05).

Conclusion: In conclusion, this study demonstrated no effect of hAM hydrogel encapsulation on the outcomes of testicular tissue transplantation.

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羊膜包封睾丸组织治疗小鼠模型的实验研究。

背景：由于缺氧导致的精原干细胞（ssc）丢失，通过睾丸组织移植恢复男性癌症个体的生育能力面临挑战。探讨了水凝胶包封以减少睾丸组织移植中的缺氧损伤。为此，人羊膜（hAM）衍生的水凝胶可能是一种替代品。目的：评价hamm水凝胶支持睾丸组织移植的潜力。材料与方法：本实验取16只雄性NMRI小鼠（4-5周，22±2 gr）睾丸组织标本（1-3 mm3）。这些组织碎片要么被包裹在来自火腿的水凝胶中，要么不被包裹（对照组），然后在接受半侧或双侧睾丸切除术的小鼠背部皮肤下进行自体移植。在第21天对移植睾丸组织进行组织学评估，包括输精管的完整性、ssc的存活、支持细胞的功能以及缺氧和凋亡。结果：各组间ST完整性、ssc数量、支持细胞功能、缺氧诱导因子1- α和凋亡率均无显著差异（p≤0.05）。结论：本研究结果表明，hAM水凝胶包封对睾丸组织移植的预后没有影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Reproductive Biomedicine OBSTETRICS & GYNECOLOGY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

16 weeks

期刊介绍： The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.