Optimizing thyroxine levels for enhanced buffalo sperm cryopreservation and fertility: a focus on quality, viability, and antioxidant protection.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-04-30 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1584903

Maha S Salama, Mohey A Ashour, Ahmed M Shehabeldin, Mohamed E A Omar, Mohamed M Soliman, Heba I Ghamry, Mohamed Abdelmegeid, Mustafa Shukry, Ahmed A Elolimy

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引用次数: 0

Abstract

Introduction: This study investigated the effects of adding thyroxine (T4) to buffalo semen on sperm quality, oxidative markers, apoptosis-like changes, and fertility.

Methods: Initially, we tested a wide range of T4 concentrations (0.1, 0.3, 0.9, 2.7, and 8.1 µg/dL) to evaluate their impact on motility and viability.

Result and discussion: Lower concentrations (0.1-0.9 µg/dL) improved total and progressive motility and viability compared to higher concentrations (2.7 and 8.1 µg/dL). We assessed optimized doses (0.25, 0.5, 0.75, and 1 µg/dL) based on these findings. The 0.75 µg/dL group showed superior sperm velocity, viability, motion parameters, membrane, acrosome, and DNA integrity in equilibrated and frozen-thawed samples. Antioxidant markers (GPx, SOD, TAC) were enhanced, while MDA and apoptotic/necrotic cell levels were reduced, particularly in the 0.75 µg/dL group. Fertility trials revealed higher cryosurvival and conception rates in thyroxine-treated groups. In conclusion, T4 supplementation, especially at 0.75 µg/dL, enhances cryopreservation outcomes and fertility potential of buffalo bull semen.

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优化甲状腺素水平，提高水牛精子冷冻保存和生育能力：关注质量，活力和抗氧化保护。

简介：本研究探讨了在水牛精液中添加甲状腺素（T4）对精子质量、氧化指标、细胞凋亡样变化和生育能力的影响。方法：最初，我们测试了大范围的T4浓度（0.1、0.3、0.9、2.7和8.1 μ g/dL），以评估它们对运动性和活力的影响。结果和讨论：与高浓度（2.7和8.1µg/dL）相比，较低浓度（0.1-0.9µg/dL）改善了总运动性和进步性和活力。我们根据这些发现评估了最佳剂量（0.25、0.5、0.75和1µg/dL）。0.75µg/dL组在平衡和冻融样品中表现出更好的精子速度、活力、运动参数、膜、顶体和DNA完整性。抗氧化标志物（GPx， SOD， TAC）增强，MDA和凋亡/坏死细胞水平降低，特别是在0.75µg/dL组。生育试验显示，甲状腺素治疗组的冷冻存活率和受孕率较高。综上所述，添加T4，特别是0.75µg/dL，可以提高水牛精液的冷冻保存效果和生育潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.