Superparamagnetic polyhemoglobin-tyrosinase nanocapsules: a novel biotherapeutic with enhanced tumor suppression with control by external magnetic field.
IF 4.3 3区 工程技术Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0
Abstract
Introduction: Our recent study shows nanobiotherepeutic Polyhemoglobin-Tyrosinase-Nanocapsules (PolyHb-Tyr-Nano) have strong anti-tumor abilities in multiple cancer lines. However, despite their tumor inhibitory potential, some internal tumor sites can be difficult to reach.
Methods: In this paper, based on Chang's original finding that artificial cells containing magnetic material can be controlled by external magnetic fields, using nanoprecipitation methods, we modified this biotechnological nanotherapeutic with superparamagnetic properties, which shown to be attracted and guided by external magnets.
Results: By fluorescence microscopy, we show that external magnetic field improved the local deposition of the nanorobotic superparamagnetic PolyHb-Tyr-nano at the tumor microenvironment (TME), significantly preventing their clearance, to stay at the tumor site despite repeated washings. This allowed time for them to enter the tumor cells to act intracellularly. In cell proliferation tests and tumor migration study, their tumor inhibitory action on the four cancer cell lines: Hepa 1-6 liver cancer line, A549 lung cancer line, HeLa cervical cancer line, and MCF7 breast cancer line are also retained effective, a low cell viability and tumor migration was observed. Furthermore, the addition of superparamagnetic property has enhanced the nanocapsules uptake and tumor inhibitory abilities, significantly improved their drug effect on tumor cells. Via cell viability test, PAL assay, oxidative stress detection, and mitochondria membrane potential studies, the PolyHb-Tyr-nano has shown improved tumor killing, by amino acid reduction, reactive oxygen species (ROS) generation, to mitochondria activity reduction in the presence of external magnetic fields.
Discussion: Our results showed the efficacy of the nanorobotic superparamagnetic PolyHb-Tyr-nano on anti-tumor effect in multiple cancer lines. This novel nanobiotherapeutic has the potential for future cancer therapy, and can enhance drug localization, targeted delivery, and combination therapies.
期刊介绍：
The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs.
In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.
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