Insight on long non-coding RNA expression profile in THP-derived macrophages infected by Mycobacterium tuberculosis H37Rv, H37Ra, and BCG.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Shima Hadifar, Abozar Ghorbani
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引用次数: 0

Abstract

Emerging evidence has suggested a potential role for long non-coding RNAs (lncRNAs) in transcriptome dysregulation during Mycobacterium tuberculosis (Mtb) infection. Understanding the regulatory functions of lncRNAs can provide further insight into the interaction between Mtb and the host. In this study, we sought to explore the lncRNA signature in the Mtb-infected THP1 macrophages (H37Rv, H37Ra, and BCG strains) using the publicly available RNA sequencing dataset of GSE162729. Our analysis identified 6202 putative lncRNAs, with the majority being novel lncRNAs, indicating their significant involvement in the Mtb-infected macrophages. We also identified several differentially expressed lncRNA genes specifically induced in each infected group. Reactome enrichment pathway analysis on cis target genes of lncRNAs revealed that inflammatory immune responses were the predominant features of lncRNAs induced during the H37Rv infection compared to H3Ra and BCG infection. Scavenging by class A receptors and inflammasomes were also highlighted as the common enriched terms among Mtb- and BCG-infected groups. Moreover, we highlighted several potential lncRNAs as hub genes in the predicted regulatory network between the differentially expressed lncRNAs and miRNAs in Mtb-infected THP-1 cells. These findings suggested a possible diverse regulatory role for lncRNAs in the macrophage response to different Mycobacterium strain infections. Further functional study of the lncRNA genes in Mtb infection, while considering the genetic background of the Mtb strain, will be a promising focus for future research.

结核分枝杆菌H37Rv、H37Ra和BCG感染的thp源性巨噬细胞中长链非编码RNA表达谱的研究
新出现的证据表明,长链非编码rna (lncRNAs)在结核分枝杆菌(Mtb)感染期间转录组失调中的潜在作用。了解lncrna的调控功能可以进一步了解Mtb与宿主之间的相互作用。在这项研究中,我们试图利用公开的RNA测序数据集GSE162729来探索mtb感染的THP1巨噬细胞(H37Rv, H37Ra和BCG株)中的lncRNA特征。我们的分析确定了6202个推测的lncrna,其中大多数是新的lncrna,表明它们在mtb感染的巨噬细胞中有重要作用。我们还鉴定了在每个感染组中特异性诱导的几个差异表达的lncRNA基因。对lncRNAs顺式靶基因的反应组富集途径分析显示,与H3Ra和BCG感染相比,H37Rv感染诱导的炎症免疫反应是lncRNAs的主要特征。A类受体和炎性小体的清除也被强调为结核分枝杆菌和bcg感染组的共同富集项。此外,我们强调了在mtb感染的THP-1细胞中差异表达的lncrna和mirna之间预测的调节网络中,几个潜在的lncrna作为枢纽基因。这些发现表明lncRNAs在巨噬细胞对不同分枝杆菌菌株感染的反应中可能具有不同的调节作用。进一步研究lncRNA基因在Mtb感染中的功能,同时考虑Mtb菌株的遗传背景,将是未来研究的一个有希望的重点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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