OsHYPK/NatA-mediated N-terminal acetylation regulates the homeostasis of NLR immune protein to fine-tune rice immune responses and growth.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2025-05-15 DOI:10.1016/j.celrep.2025.115719

Yaqian Huang, Xiaodi Gong, Hui Shi, Peiyi Wang, Yundong Yuan, Cuilian Kong, Jie Zhou, Dianxing Wu, Yan Liang, Yonghong Wang, Jing Wang

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引用次数: 0

Abstract

Keeping nucleotide-binding leucine-rich repeat (NLR) protein at appropriate levels is critical for plant survival. Huntingtin Yeast Partner K (OsHYPK) was previously identified as a positive regulator of N-terminal acetyltransferase A (NatA) activity in rice. Here, we find that oshypk shows enhanced resistance to Magnaporthe oryzae (M. oryzae). Through screening for suppressors of oshypk (soh), we identify suppressor soh74, which contains a mutation in RESISTANCE TO P. SYRINGAE PV MACULICOLA1 (RPM1)-like NLR protein (RPM1-L1) and exhibits compromised resistance to M. oryzae. Mechanistically, declined N-terminal acetylation (NTA) degree in oshypk leads to protein accumulation of RPM1-L1, contributing to enhanced disease resistance. To restrict RPM1-L1 accumulation, OsHYPK is expressed at high levels under normal conditions. However, pathogen infection reduces OsHYPK level to release the inhibition on RPM1-L1, leading to immune activation. This study reveals a vital pathway in which OsHYPK/NatA-mediated NTA rapidly fine-tunes NLR-mediated immune response.

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OsHYPK/ nata介导的n端乙酰化调节NLR免疫蛋白的稳态，调控水稻的免疫应答和生长。

保持核苷酸结合丰富亮氨酸重复序列（NLR）蛋白的适当水平对植物的生存至关重要。亨廷顿酵母伴侣K （OsHYPK）是水稻n端乙酰转移酶a （NatA）活性的正调节因子。本研究发现，oshypk对稻瘟病菌（Magnaporthe oryzae, m.o ryzae）的抗性增强。通过筛选oshypk （soh）的抑制因子，我们发现了soh74抑制因子，该抑制因子含有一个突变的抗P. SYRINGAE PV MACULICOLA1 （RPM1）样NLR蛋白（RPM1- l1），并表现出对M. oryzae的抗性受损。从机制上讲，oshypk中n端乙酰化（NTA）程度的下降导致RPM1-L1蛋白的积累，有助于增强抗病性。为了限制RPM1-L1的积累，OsHYPK在正常情况下高水平表达。然而，病原体感染降低OsHYPK水平，释放对RPM1-L1的抑制，导致免疫激活。这项研究揭示了OsHYPK/ nata介导的NTA快速微调nlr介导的免疫反应的重要途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.