A Study on the Anti-Inflammatory and Antibacterial Effects of Huanglian Jiedu Decoction in the Treatment of Pressure Injuries.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Combinatorial chemistry & high throughput screening Pub Date : 2025-05-13 DOI:10.2174/0113862073240207250424192712

Jing Gao, Dingxi Bai, Wenting Ji, Lin Zhu, Xianying Lu, Wei Wang, Chaoming Hou

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引用次数: 0

Abstract

Objective: Pressure Injuries (PIs) severely affect the quality of life of patients. Infection and inflammation are key factors contributing to the progression of pressure injuries; therefore, their inhibition plays a crucial role in preventing the worsening of the condition. Huanglian Jiedu Decoction (HLJDD), as a typical traditional Chinese medicine with heat-clearing and detoxicating effects, has good broad-spectrum antibacterial and anti-inflammatory effects. It is commonly used in the treatment of clinically infected external wounds. However, the therapeutic effects of HLJDD on PI remains unclear.

Methods: The extract of HLJDD was prepared using the water extraction and alcohol precipitation method. Sixty male SD rats were randomly divided into five groups (n = 12/group): control group, model group, normal saline group (negative control group), iodophor group (positive control group), and HLJDD group (test group). Except for the control group, magnet clamping and the Staphylococcus aureus inoculation method were used to construct the model of stage 3 PI infection wound in the other groups. After irrigating the wound, the healing rate, bacterial concentration, concentrations of IL-1, IL-6, and TNFα, and the protein expression levels of TLR2, MyD88, and NF-Bp65 were examined. Skin and ocular mucosal irritation tests were conducted to evaluate the safety of the topical application of HLJDD.

Results: Rats treated with HLJDD exhibited an improved wound healing rate, along with reduced bacterial concentration on the wound surface and a significant decrease in the content of inflammatory cytokines (IL-1β, IL-6, and TNF-α). The protein expression levels of TLR2, MyD88, and NF-κBp65 were down-regulated after the administration of HLJDD. The prepared HLJDD did not cause any irritation.

Conclusion: HLJDD can promote the healing of PI wounds and has a protective effect on PI through its anti-inflammatory and anti-bacterial properties.

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黄连解毒汤治疗压伤抗炎抗菌作用的研究。

目的：压伤严重影响患者的生活质量。感染和炎症是导致压伤进展的关键因素；因此，它们的抑制在防止病情恶化方面起着至关重要的作用。黄连解毒汤作为典型的清热解毒中药，具有良好的广谱抗菌和抗炎作用。常用于临床感染性外伤的治疗。然而，HLJDD对PI的治疗效果尚不清楚。方法：采用水提醇沉法制备HLJDD提取物。雄性SD大鼠60只，随机分为5组（n = 12/组）：对照组、模型组、生理盐水组（阴性对照组）、碘伏组（阳性对照组）、HLJDD组（试验组）。除对照组外，其余各组均采用磁铁夹持法和金黄色葡萄球菌接种法构建PI 3期感染创面模型。冲洗创面后，检测创面愈合率、细菌浓度、IL-1、IL-6、tnf - α浓度及TLR2、MyD88、NF-Bp65蛋白表达水平。通过皮肤和眼粘膜刺激试验来评价HLJDD外用的安全性。结果：经HLJDD处理的大鼠伤口愈合率提高，伤口表面细菌浓度降低，炎症因子（IL-1β、IL-6和TNF-α）含量显著降低。给药后，TLR2、MyD88、NF-κBp65蛋白表达水平下调。制备的HLJDD无任何刺激性。结论：HLJDD可促进PI创面愈合，并通过其抗炎、抗菌作用对PI创面具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.