LC-MS/MS determination of trazodone in human plasma and its pharmacokinetic study in healthy Chinese.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-05-15 DOI:10.1080/17576180.2025.2506349

Kaiwei Luo, Danrui Liu, Shengshuo Zhang, Xin Zhang, Qunan Cheng, Jie Lin, Yonghua Yu

引用次数: 0

Abstract

Aims: This study aimed to establish a robust LC-MS/MS method for quantifying trazodone in human plasma and investigate its pharmacokinetic profile in healthy Chinese subjects under fasting and fed conditions.

Materials & methods: A validated LC-MS/MS method using protein precipitation was applied to analyze trazodone in 68 healthy subjects (34 fasting/34 fed), with full method validation performed.

Results: This method exhibited high selectivity, precision, and accuracy. The results showed good linearity in the range of 5-3000 ng/mL and matrix effect results showed no matrix interference. The pharmacokinetic data revealed notable differences between the fasting and postprandial states.

Conclusions: The validated LC-MS/MS method proved reliable for trazodone quantification. The observed food effects on absorption suggest clinical dosing should consider administration conditions to ensure optimal therapeutic outcomes.

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人血浆中曲唑酮的LC-MS/MS测定及其在健康人群中的药动学研究。

目的：建立一种高效液相色谱-质谱联用方法定量测定人血浆中曲唑酮的含量，并研究其在空腹和空腹条件下的药代动力学特征。材料与方法：采用经验证的LC-MS/MS蛋白质沉淀法对68名健康受试者（34名禁食/34名喂养）的曲唑酮进行分析，并进行了完整的方法验证。结果：该方法选择性高，精密度高，准确度高。在5 ~ 3000 ng/mL范围内线性良好，基质效应无干扰。药代动力学数据显示空腹和餐后状态之间存在显著差异。结论：经验证的LC-MS/MS方法可用于曲唑酮的定量。观察到的食物对吸收的影响提示临床给药应考虑给药条件以确保最佳治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.