Integrated metabolome and immunity analysis of immune-physiological responses in dairy cows under heat stress condition.

IF 2.5 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2025-10-01 Epub Date: 2025-05-12 DOI:10.5713/ab.25.0038
Jun Sik Eom, Sangjin Lee, Joonpyo Oh, Byeong Cheol Ban, Yeeun Kim, Goeun Han, Bon-Hee Gu, Eun-Tae Kim, Sang-Bum Kim, Sung Sill Lee, Myunghoo Kim
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引用次数: 0

Abstract

Objective: This study aimed to investigate the metabolic changes and immune responses in Jersey dairy cows under heat stress (HS). The focus was on understanding HS-induced alterations in metabolism and immunity compared to HS-free cows.

Methods: Proton nuclear magnetic resonance spectroscopy-based metabolomics was performed on rumen fluid, serum, and milk samples which were collected through single sampling from HS-free (n = 9) and HS-exposed (n = 8) Jersey cows. Immune cell proportions and functions in peripheral blood mononuclear cells (PBMC) were analyzed to identify heat-sensitive immunological changes. Correlation analysis was conducted to link immune indicators with serum metabolites.

Results: Metabolomics revealed potential HS biomarkers in biofluids: nicotinate and phenylacetate in rumen fluid; isopropanol in serum; and glycine and trehalose in milk. HS increased B cell and CD4+ T cell populations, as well as Th17 cells and IL-17A expression in PBMCs. Twenty-three metabolites correlated strongly with five immune indicators, with nine metabolites exhibiting a positive correlation and fourteen a negative correlation.

Conclusion: HS alters the both metabolism and immune cell function in Jersey dairy cows. These findings provide key information for the development of diagnostic methods for HS and nutritional intervention strategies to mitigate HS for dairy cows.

热应激条件下奶牛免疫生理反应的综合代谢组学和免疫分析。
目的:研究热应激条件下泽西奶牛的代谢变化和免疫反应。重点是了解与不含hs的奶牛相比,hs诱导的代谢和免疫变化。方法:采用质子核磁共振代谢组学方法,对无hs (n = 9)和hs暴露(n = 8)泽西奶牛的瘤胃液、血清和乳样品进行单次采样。分析外周血单核细胞的免疫细胞比例和功能,以确定热敏感的免疫变化。对免疫指标与血清代谢物进行相关性分析。结果:代谢组学揭示了生物体液中潜在的HS生物标志物:瘤胃液中的烟酸盐和苯乙酸盐;血清异丙醇;牛奶中的甘氨酸和海藻糖。HS增加了pbmc中B细胞和CD4+ T细胞数量,以及Th17细胞和IL-17A的表达。23种代谢物与5项免疫指标呈强相关,其中9种代谢物呈正相关,14种呈负相关。结论:热应激改变了泽西奶牛的代谢和免疫细胞功能。这些发现为奶牛热应激诊断方法的发展和缓解奶牛热应激的营养干预策略提供了重要信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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