Effect of inhibiting prolactin secretion on secondary hair follicle development in cashmere goats.

IF 2.4 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Bioscience Pub Date : 2025-05-12 DOI:10.5713/ab.25.0053

Chunhui Duan, Xuejiao Yin, Kexing Hao, Lechao Zhang, Yuchun Xie, Xueru Li, Yueqin Liu, Yingjie Zhang

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Abstract

Objective: This study aims to investigate the molecular mechanisms of inhibiting prolactin (PRL) secretion on secondary hair follicle (SHF) development in cashmere goats.

Methods: A total of 20 cashmere goats were randomly assigned to either a bromocriptine (PRL inhibitor, BCT, 0.06 mg/kg BW) treatment (BCT+) or a control (BCT-) group. Blood and skin samples were collected monthly in telogen, and cashmere samples were collected after the cashmere grew. Furthermore, the dermal papilla cells (DPCs) were isolated from SHF and cultured with PRL.

Results: The results showed that the percentage of active SHF in telogen was decreased (p < 0.05) in the BCT+ group. The inhibition of PRL secretion reduced (p < 0.05) the serum PRL concentration, and the expression of the PRL, SPRLR, Kit, and Fos genes. Transcriptome analysis of skin tissues identified differentially expressed genes. The results of the in vitro experiment indicated that 150 ng/mL PRL promoted (p < 0.05) the proliferation and migration of DPCs.

Conclusion: The Kit gene mediates PRL's regulation of the activation of SHF by stimulating the activation of Fos. It demonstrated that inhibiting PRL secretion in telogen could reduce the number of activated SHF and hair bulb widths.

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抑制泌乳素分泌对绒山羊次生毛囊发育的影响。

目的：探讨抑制泌乳素（PRL）分泌对绒山羊次生毛囊发育的分子机制。方法：选取20只绒山羊，随机分为溴隐亭（PRL抑制剂，BCT, 0.06 mg/kg BW）处理组（BCT+）和对照组（BCT-）。休止期每月采集血液和皮肤样本，羊绒生长后采集羊绒样本。此外，从SHF中分离出真皮乳头细胞（DPCs），用PRL培养。结果：BCT+组小鼠静止期活跃SHF百分比降低（p < 0.05）。抑制PRL分泌可降低血清PRL浓度（p < 0.05），降低PRL、SPRLR、Kit和Fos基因表达（p < 0.05）。皮肤组织转录组分析鉴定出差异表达基因。体外实验结果表明，150 ng/mL PRL对DPCs的增殖和迁移有促进作用（p < 0.05）。结论：Kit基因通过刺激Fos的活化介导PRL对SHF活化的调控。结果表明，在休止期抑制PRL分泌可减少激活的SHF数量和毛球宽度。

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