Rabbit Induced Pluripotent Stem Cells -Derived Mesenchymal Stem Cells for Enhanced Wound Healing.

IF 4 2区医学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

STEM CELLS Pub Date : 2025-05-16 DOI:10.1093/stmcls/sxaf028

Hsing-Yi Yu, Yang-Zhe Huang, Edward Chern

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Abstract

Mesenchymal stem cells (MSCs) are pivotal in regenerative medicine, particularly for their efficacy in tissue repair. However, sourcing high-quality MSCs presents challenges due to limited availability and compromised function. Induced pluripotent stem cells (iPSCs) offer a promising alternative for generating MSCs through specific differentiation protocols. In this study, we employed rabbit iPSCs to explore their capacity for differentiation into MSCs, facilitated by the use of SB431542, a TGF-β signaling inhibitor. Upon treatment with SB431542, rabbit iPSCs underwent embryoid body (EB) formation, leading to successful differentiation into the mesenchymal lineage. Our results demonstrated significant upregulation of mesodermal markers while reduced expression of ectodermal and endodermal markers, confirming effective MSC differentiation. Additionally, in a mouse wound healing model, rabbit iPSC-derived MSCs significantly enhanced wound closure compared to controls. These findings highlight the potential of SB431542 in generating functional iPSC-derived MSCs, offering valuable applications in regenerative medicine across species.

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兔诱导多能干细胞衍生间充质干细胞促进伤口愈合。

间充质干细胞（MSCs）在再生医学中起着关键作用，特别是在组织修复方面。然而，由于可用性有限和功能受损，采购高质量的msc面临挑战。诱导多能干细胞（iPSCs）通过特定的分化方案为生成间充质干细胞提供了一个有希望的替代方案。在这项研究中，我们利用兔iPSCs来探索它们在TGF-β信号抑制剂SB431542的促进下向MSCs分化的能力。经SB431542处理后，兔iPSCs形成胚状体（EB），成功分化为间充质细胞系。我们的研究结果显示，中胚层标记物显著上调，而外胚层和内胚层标记物的表达减少，证实了间充质干细胞的有效分化。此外，在小鼠伤口愈合模型中，与对照组相比，兔ipsc衍生的MSCs显著增强了伤口愈合。这些发现强调了SB431542在生成功能性ipsc来源的MSCs方面的潜力，为跨物种再生医学提供了有价值的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

STEM CELLS 医学-生物工程与应用微生物

CiteScore

10.30

自引率

1.90%

发文量

104

审稿时长

3 months

期刊介绍： STEM CELLS, a peer reviewed journal published monthly, provides a forum for prompt publication of original investigative papers and concise reviews. STEM CELLS is read and written by clinical and basic scientists whose expertise encompasses the rapidly expanding fields of stem and progenitor cell biology. STEM CELLS covers: Cancer Stem Cells, Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells, Regenerative Medicine, Stem Cell Technology: Epigenetics, Genomics, Proteomics, and Metabonomics, Tissue-Specific Stem Cells, Translational and Clinical Research.