Antimicrobial 3D printed gelatin scaffolds for root canal disinfection in regenerative endodontics procedures.

IF 5.8 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Mateo Dallos Ortega, Jenny Aveyard, Raghda Magdy Abdelgawad, Reem El-Gendy, Alexander Ciupa, David Whetnall, Julia Behnsen, Robert J Poole, Raechelle A D'Sa
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引用次数: 0

Abstract

Regenerative endodontic procedures (REPs) which aim to promote root development and pulp tissue regeneration in necrotic immature teeth, have emerged as a promising therapeutic approach. A critical determinant of REP success hinges on effective disinfection of the root canal system, which must eliminate microbial contaminants whilst preserving the microenvironment necessary for dental pulp stem cell tissue regeneration. This study reports on the fabrication of biocompatible 3D printed hydrogel scaffolds designed for root canal disinfection. The scaffolds incorporate benzyldimethyldodecylammonium chloride (BDMDAC) a broad-spectrum quaternary ammonium compound characterised by low cytotoxicity and minimal risk of resistance development. BDMDAC loaded gelatin biomaterial inks were systematically evaluated for rheology properties, mechanical stability and drug release properties. Scaffolds containing 150 μg mL-1 and 250 μg mL-1 BDMDAC exhibited excellent antimicrobial efficacy against 5 bacterial pathogens (including 3 endodontic bacteria-Enterococcus faecalis, Porphyromonas gingivalis, and Streptococcus mutans). Cytocompatibility assays using primary human dental pulp stem cells (HDPSCs) derived from 3 donors confirmed over 70% of cell viability. Furthermore, freeze-dried scaffolds demonstrated excellent shelf-life stability for at least six months. Overall, these findings highlight the potential of 3D printed BDMDAC-loaded 3D printed gelatin scaffolds as an effective and cytocompatible platform for root canal disinfection in REPs.

抗菌3D打印明胶支架根管消毒再生牙髓治疗程序。
再生牙髓治疗(REPs)旨在促进坏死未成熟牙齿的根发育和牙髓组织再生,已成为一种很有前途的治疗方法。REP成功的一个关键决定因素取决于根管系统的有效消毒,这必须消除微生物污染物,同时保留牙髓干细胞组织再生所必需的微环境。本研究报道了用于根管消毒的生物相容性3D打印水凝胶支架的制备。该支架含有苯基二甲基十二烷基氯化铵(BDMDAC),这是一种广谱季铵化合物,具有低细胞毒性和最小的耐药风险。系统评价了BDMDAC负载明胶生物材料墨水的流变性能、机械稳定性和药物释放性能。含有150 μg mL-1和250 μg mL-1 BDMDAC的支架对5种细菌病原体(包括3种牙髓细菌-粪肠球菌、牙龈卟啉单胞菌和变形链球菌)具有良好的抗菌效果。使用来自3个供体的原代人牙髓干细胞(HDPSCs)进行细胞相容性测定,证实细胞存活率超过70%。此外,冻干支架表现出至少6个月的优异货架寿命稳定性。总的来说,这些发现突出了3D打印bdmdac负载的3D打印明胶支架作为REPs根管消毒的有效和细胞兼容平台的潜力。
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来源期刊
Biomaterials Science
Biomaterials Science MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.50%
发文量
556
期刊介绍: Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.
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