Localized Light-Triggered CO Delivery: Comparing the Amount of CO Delivered and Cellular Toxicity.

IF 3.5 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

ACS Chemical Biology Pub Date : 2025-05-14 DOI:10.1021/acschembio.4c00884

C Taylor Dederich, Livia S Lazarus, Abby D Benninghoff, Lisa M Berreau

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Abstract

Molecules that enable the controlled delivery of carbon monoxide (CO) in biological environments are of significant current interest to probe the beneficial roles of CO for humans. Assumptions regarding the ability of molecules to reliably deliver CO continue to impact the field, including in work involving non-metal CO delivery motifs. Flavonols are drawing increasing interest as light-triggered CO release motifs due to their ease of synthesis, functionalization, and fluorescence trackability. Importantly, the light-driven CO release properties of flavonols depend on their structure and must be fully evaluated under various conditions to understand the relationship between the amount of CO delivered and the induced biological effects. Herein, we use a family of amine-functionalized π-extended flavonols to demonstrate that structural differences result in differing interactions with biomolecules, cellular uptake, and changes in subcellular localization, which can affect the amount of CO delivered intracellularly. This results in differences in the CO-induced cellular toxicity.

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局部光触发CO递送：比较CO递送量和细胞毒性。

能够在生物环境中控制一氧化碳（CO）递送的分子是当前探索CO对人类有益作用的重要兴趣。关于分子可靠输送CO的能力的假设继续影响着该领域，包括涉及非金属CO输送基序的工作。黄酮醇由于其易于合成、功能化和荧光可追踪性，作为光触发CO释放基元而引起越来越多的兴趣。重要的是，黄酮醇的光驱动CO释放特性取决于其结构，必须在各种条件下进行充分评估，以了解CO释放量与诱导的生物效应之间的关系。在此，我们使用一个胺功能化π扩展黄酮醇家族来证明结构差异导致与生物分子的不同相互作用，细胞摄取和亚细胞定位的变化，从而影响CO在细胞内传递的量。这导致了co诱导的细胞毒性的差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Chemical Biology 生物-生化与分子生物学

CiteScore

7.50

自引率

5.00%

发文量

353

审稿时长

3.3 months

期刊介绍： ACS Chemical Biology provides an international forum for the rapid communication of research that broadly embraces the interface between chemistry and biology. The journal also serves as a forum to facilitate the communication between biologists and chemists that will translate into new research opportunities and discoveries. Results will be published in which molecular reasoning has been used to probe questions through in vitro investigations, cell biological methods, or organismic studies. We welcome mechanistic studies on proteins, nucleic acids, sugars, lipids, and nonbiological polymers. The journal serves a large scientific community, exploring cellular function from both chemical and biological perspectives. It is understood that submitted work is based upon original results and has not been published previously.