Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-05-16 DOI:10.1002/bmc.70115

Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng

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引用次数: 0

Abstract

Gardeniae Fructus (GF) has been used as a hepatoprotective medicine; however, the active ingredients of GF against cholestatic liver injury (CLI) remain unclear. This study aims to explore active ingredients of the hepatoprotective effects of raw GF (RGF) and stir-baked GF (SGF) by spectrum–effect relationship analysis. A total of 32 common peaks were recorded in RGF and SGF HPLC fingerprints, and nine of them were structurally characterized. Both RGF and SGF demonstrated hepatoprotective effects in an ANIT-induced CLI rat model. The spectrum–effect relationship analysis results showed that peaks 3 (gardenoside), 4, 5 (jasminoside B), 6 (genipin 1-gentiobioside), 8 (geniposide), 9, 16, 17, 19, and 22 were determined as the potential active ingredients of GF against CLI. Notably, gardenoside, jasminoside B, and genipin 1-gentiobioside exhibited good hepatoprotective effects. The research establishes a research foundation for the future quality control and medicinal application of GF.

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基于谱效关系分析的生炒栀子保肝活性成分研究

栀子果（GF）已被用作肝保护药物；然而，GF抗胆汁淤积性肝损伤（CLI）的有效成分尚不清楚。本研究旨在通过谱效关系分析，探讨生GF （RGF）和炒GF （SGF）的保肝活性成分。RGF和SGF HPLC指纹图谱共记录到32个共同峰，其中9个峰进行了结构表征。RGF和SGF在anit诱导的CLI大鼠模型中均表现出肝脏保护作用。谱效关系分析结果表明，峰3（栀子苷）、峰4、峰5（栀子苷B）、峰6（栀子苷1-栀子苷）、峰8（栀子苷）、峰9、峰16、峰17、峰19、峰22为GF抗CLI的潜在有效成分。其中栀子苷、栀子苷B和龙胆素1-龙胆苷表现出良好的肝保护作用。本研究为今后GF的质量控制和药用奠定了研究基础。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.