In Vivo Model of Bone Regeneration with Aspirin Cross-linked O-carboxymethyl Chitosan Hydrogel Loaded with Adipose Derived-Stem Cells

IF 4.7 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL

Journal of Polymers and the Environment Pub Date : 2025-04-26 DOI:10.1007/s10924-025-03571-y

Simzar Hosseinzadeh, Lida Shahghasempour, Hojjat Allah Abbaszadeh, Masoud Soleimani, Mohammad Amin Abdollahifar, Mehdi Rasouli, Mohammad Mehdi Khani, Roya Fattahi

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引用次数: 0

Abstract

Aspirin, also known as acetylsalicylic acid (ASA), a hydrophilic substance, was selected as a cross-linker to create the three-dimensional structure of the chitosan hydrogel. The main objective of this research is to incorporate aspirin as a crosslinker in hydrogels to enhance bone regeneration. O-carboxymethyl chitosan (O-CMC) was synthesized. The 0.09 O-CMC hydrogel was prepared and mixed with an aspirin solution at a concentration of 0.05 g/ml. The resulting gel composite O-CMC/ASA with and without human adipose-derived stem cell (ADSC) was then used to fill 8-mm skull defects in the Wistar model for 4 and 8 weeks, enabling an evaluation of its potential to stimulate bone formation in vivo. Histological examination and CT revealed that the hydrogel O-CMC/ASA/ADSCs promoted the migration and proliferation of ADSCs, leading to a significant increase in new bone formation compared with hydrogel O-CMC.

Graphical Abstract

Optimization process of PVDF gradient unit cell lattice structures for Additive Manufacturing

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阿司匹林交联o -羧甲基壳聚糖水凝胶负载脂肪干细胞骨再生的体内模型

阿司匹林，也被称为乙酰水杨酸（ASA），是一种亲水性物质，被选择作为交联剂来创建壳聚糖水凝胶的三维结构。本研究的主要目的是将阿司匹林作为交联剂纳入水凝胶中，以增强骨再生。合成了o -羧甲基壳聚糖（O-CMC）。制备0.09 O-CMC水凝胶，与浓度为0.05 g/ml的阿司匹林溶液混合。然后用含和不含人脂肪源性干细胞（ADSC）的O-CMC/ASA凝胶复合材料填充Wistar模型中8mm的颅骨缺损4周和8周，从而评估其在体内刺激骨形成的潜力。组织学检查和CT显示，水凝胶O-CMC/ASA/ADSCs促进了ADSCs的迁移和增殖，与水凝胶O-CMC相比，新骨形成明显增加。面向增材制造的PVDF梯度单元格结构优化过程

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.