Field Marigold (Calendula arvensis L.) accelerates wound-healing in vivo: role of transforming growth factor-beta1 (TGF-β1), inflammatory, and biochemical molecules

IF 2.9 4区 生物学 Q3 CELL BIOLOGY
Safwan Mahmoud Al-Adwan, Talal Salem Al-Qaisi, Ahmed A.j. Jabbar, Kamaran Younis M. Amin, Hawri Fatih Sami, Hanan Ibrahim Althagbi, Ahmed Hameed Al-Dabhawi, Bassam Ali Abed Wahab, Rawaz Rizgar Hassan, Mahmood Ameen Abdulla, Musher Ismael Saleh
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引用次数: 0

Abstract

Medicinal plants are major sources of natural products, which have gained a renewed interest in recent years because of drawbacks associated with synthetic ones used for human health disorders. Calendula arvensis L. is a traditional medicinal plant used for many inflammatory-related diseases. The study explores the acute toxicity and wound-healing effects of methanolic extracts of Calendula arvensis L. aerial parts (MECAA) on excisional neck injury in rats. A two-week acute toxicity procedure was applied to find the safety of MECAA in animal models. For the wound-healing experiment, a uniform dorsal neck injury was created for twenty-four Sprague–Dawley rats, which were aligned arbitrarily into 4 groups and received topical treatments; group A rats addressed with normal saline; group B rats had intrasite gel; groups C and D rats had 0.2 ml of 250 and 500 mg/kg of MECAA, respectively. There was no toxicity nor mortality in rats supplemented orally with up to 5 g/kg MECAA. MECAA topical addressing accelerated wound contraction represented by higher deposition of fibroblast and keratinocytes, angiogenic factors, and reduced inflammatory cells. MECAA treatment up-regulated tissue antioxidants (SOD and CAT), transforming growth factor-β 1, and hydroxyproline (collagen) contents while lowering MDA and serum inflammatory mediators (TNF-α and IL-6). The outcomes can serve as scientific evidence for the traditional use of Calendula arvensis as a wound healer, which requires further molecular isolation and identification as a viable source of a potent drug formulation.

野金盏花(Calendula arvensis L.)在体内加速伤口愈合:TGF-β1、炎症和生化分子的作用
药用植物是天然产物的主要来源,近年来,由于用于人类健康失调的合成产物存在缺陷,天然产物重新引起人们的兴趣。金盏菊(Calendula arvensis L.)是一种传统的药用植物,用于治疗许多炎症相关疾病。本研究探讨金盏菊(Calendula arvensis L. aerial parts,简称MECAA)甲醇提取物对大鼠切除性颈部损伤的急性毒性及创面愈合作用。采用两周急性毒性实验验证MECAA在动物模型中的安全性。创面愈合实验,取24只sd大鼠制作均匀背颈损伤,随机分为4组,局部治疗;A组大鼠灌胃生理盐水;B组大鼠静脉注射凝胶;C、D组大鼠分别给予250、500 mg/kg MECAA 0.2 ml。口服5 g/kg MECAA对大鼠无毒性,也无死亡。MECAA局部处理加速伤口收缩,表现为成纤维细胞和角化细胞、血管生成因子的沉积增加,炎症细胞减少。MECAA处理上调组织抗氧化剂(SOD和CAT)、转化生长因子-β 1和羟脯氨酸(胶原)含量,降低MDA和血清炎症介质(TNF-α和IL-6)。这些结果可以作为金盏菊作为伤口愈合剂的传统用途的科学证据,这需要进一步的分子分离和鉴定,作为有效药物制剂的可行来源。
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来源期刊
Journal of Molecular Histology
Journal of Molecular Histology 生物-细胞生物学
CiteScore
5.90
自引率
0.00%
发文量
68
审稿时长
1 months
期刊介绍: The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.
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