Field Marigold (Calendula arvensis L.) accelerates wound-healing in vivo: role of transforming growth factor-beta1 (TGF-β1), inflammatory, and biochemical molecules
Safwan Mahmoud Al-Adwan, Talal Salem Al-Qaisi, Ahmed A.j. Jabbar, Kamaran Younis M. Amin, Hawri Fatih Sami, Hanan Ibrahim Althagbi, Ahmed Hameed Al-Dabhawi, Bassam Ali Abed Wahab, Rawaz Rizgar Hassan, Mahmood Ameen Abdulla, Musher Ismael Saleh
{"title":"Field Marigold (Calendula arvensis L.) accelerates wound-healing in vivo: role of transforming growth factor-beta1 (TGF-β1), inflammatory, and biochemical molecules","authors":"Safwan Mahmoud Al-Adwan, Talal Salem Al-Qaisi, Ahmed A.j. Jabbar, Kamaran Younis M. Amin, Hawri Fatih Sami, Hanan Ibrahim Althagbi, Ahmed Hameed Al-Dabhawi, Bassam Ali Abed Wahab, Rawaz Rizgar Hassan, Mahmood Ameen Abdulla, Musher Ismael Saleh","doi":"10.1007/s10735-025-10433-3","DOIUrl":null,"url":null,"abstract":"<div><p>Medicinal plants are major sources of natural products, which have gained a renewed interest in recent years because of drawbacks associated with synthetic ones used for human health disorders. <i>Calendula arvensis</i> L. is a traditional medicinal plant used for many inflammatory-related diseases. The study explores the acute toxicity and wound-healing effects of methanolic extracts of <i>Calendula arvensis</i> L. aerial parts (MECAA) on excisional neck injury in rats. A two-week acute toxicity procedure was applied to find the safety of MECAA in animal models. For the wound-healing experiment, a uniform dorsal neck injury was created for twenty-four Sprague–Dawley rats, which were aligned arbitrarily into 4 groups and received topical treatments; group A rats addressed with normal saline; group B rats had intrasite gel; groups C and D rats had 0.2 ml of 250 and 500 mg/kg of MECAA, respectively. There was no toxicity nor mortality in rats supplemented orally with up to 5 g/kg MECAA. MECAA topical addressing accelerated wound contraction represented by higher deposition of fibroblast and keratinocytes, angiogenic factors, and reduced inflammatory cells. MECAA treatment up-regulated tissue antioxidants (SOD and CAT), transforming growth factor-β 1, and hydroxyproline (collagen) contents while lowering MDA and serum inflammatory mediators (TNF-α and IL-6). The outcomes can serve as scientific evidence for the traditional use of <i>Calendula arvensis</i> as a wound healer, which requires further molecular isolation and identification as a viable source of a potent drug formulation.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 3","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Molecular Histology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10735-025-10433-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Medicinal plants are major sources of natural products, which have gained a renewed interest in recent years because of drawbacks associated with synthetic ones used for human health disorders. Calendula arvensis L. is a traditional medicinal plant used for many inflammatory-related diseases. The study explores the acute toxicity and wound-healing effects of methanolic extracts of Calendula arvensis L. aerial parts (MECAA) on excisional neck injury in rats. A two-week acute toxicity procedure was applied to find the safety of MECAA in animal models. For the wound-healing experiment, a uniform dorsal neck injury was created for twenty-four Sprague–Dawley rats, which were aligned arbitrarily into 4 groups and received topical treatments; group A rats addressed with normal saline; group B rats had intrasite gel; groups C and D rats had 0.2 ml of 250 and 500 mg/kg of MECAA, respectively. There was no toxicity nor mortality in rats supplemented orally with up to 5 g/kg MECAA. MECAA topical addressing accelerated wound contraction represented by higher deposition of fibroblast and keratinocytes, angiogenic factors, and reduced inflammatory cells. MECAA treatment up-regulated tissue antioxidants (SOD and CAT), transforming growth factor-β 1, and hydroxyproline (collagen) contents while lowering MDA and serum inflammatory mediators (TNF-α and IL-6). The outcomes can serve as scientific evidence for the traditional use of Calendula arvensis as a wound healer, which requires further molecular isolation and identification as a viable source of a potent drug formulation.
期刊介绍:
The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes.
Major research themes of particular interest include:
- Cell-Cell and Cell-Matrix Interactions;
- Connective Tissues;
- Development and Disease;
- Neuroscience.
Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance.
The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.