Simultaneous Release of Hydrophilic and Hydrophobic Drugs by a pH-sensitive Bio-carrier Based on Layered Double Hydroxides Decorated with l-serine-chitosan
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Abstract
Recently, the development of pH-responsive carriers has emerged as a promising area of research in cancer treatment. For these reasons, the purpose of the present work is to investigate layered double hydroxides decorated with l-serine-chitosan (LDH-DOX-CUR/Ser-CS) for the targeted release of doxorubicin (DOX, hydrophilic) and curcumin (CUR, hydrophobic) drugs to HepG2 liver cancer cells. Different methods such as FT-IR, FE-SEM, Zeta potential, EDX, and XRD analysis were employed to validate the structural properties of the developed bio-carriers. The encapsulation efficiencies for DOX and CUR were ∼96.8% and ∼83.6%, respectively. The drug release evaluation highlighted the pH-sensitive and controlled release capabilities of the LDH-DOX-CUR/Ser-CS bio-carriers to acidic tumor microenvironments. Both drugs’ release mechanisms also showed compliance with the Fickian diffusion from the Korsmeyer-Peppas model. Additionally, the bioavailability of the designed LDH against both HepG2 (liver cancer cell line) and L929 (normal liver cell line) cells was proved by cytotoxicity test. On the other hand, the LDH-DOX-CUR/Ser-CS bio-carriers exhibited higher cytotoxicity against HepG2 cells, which is caused by the controlled and targeted delivery of both drugs to these cells. The antibacterial, antioxidant, and blood compatibility properties of the engineered bio-carriers were confirmed by relevant in vitro techniques. In general, the results of this study showed that engineered bio-carriers have the necessary potential and efficiency for use as drug delivery bio-carriers in the biomedical field.
期刊介绍：
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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