Molecular Characterization of Paragonimus westermani from South India

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2025-05-16 DOI:10.1007/s11686-025-01044-9

Anisha Joy, Anju Varghese, Chundayil Kalarickal Deepa, Karapparambu Gopalan Ajith Kumar, Sebasteena Peekunnel Francis, Aleena Iype, Geethu Reghu, Sreenidhi Nellikka Chalapron, Anaswara Aravindhakshan, George Chandy, Pradeep Mampillikalam, Hamza Palekkodan, Reghu Ravindran

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Abstract

Purpose

Paragonimus westermani is the most common lung fluke infecting humans in Asia. This study aimed to molecularly characterize adult P. westermani from the south India (Kerala) using ITS-2, partial 28 S rDNA and partial cox1 gene sequences.

Methods

Adult flukes were recovered from the lungs of a tiger (Panthera tigris) (n = 10), a jungle cat (Felis chaus) (n = 6) and a domesticated dog (Canis familiaris) (n = 4). ITS-2, partial 28 SrDNA and partial cox1 gene sequences were amplified by polymerase chain reaction and a concatenated phylogenetic tree was constructed with these three marker sequences. Intra-species and inter-species genetic distances were calculated, and haplotype network analysis was performed using partial cox1 gene.

Results

Phylogenetic analysis based on the concatenated tree showed that Kerala isolates formed P. westermani complex with other isolates of P. westermani from India and from other countries. The Kerala isolates were genetically closer to metacercaria morphotype 2 of P. westermani. However, the genetic distance was higher among Indian isolates, indicating significant genetic variation among P. westermani within the complex. In addition, India also exhibited the highest number of haplotypes (n = 8).

Conclusions

Concatenated phylogenetic analysis of the Indian isolates of P. westermani show them to form P. westermani complex. The parasite exhibits high genetic distance with a large number of haplotypes. Further epidemiological studies are needed to understand its genetic variability. No sub-populations have been recognized in P. westermani based on the presented molecular data.

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南印度卫氏并殖吸虫的分子特征

目的威氏吸虫是亚洲地区最常见的人类肺部吸虫。本研究旨在利用ITS-2、部分28s rDNA和部分cox1基因序列对来自印度南部喀拉拉邦的成年P. westermani进行分子表征。方法分别从虎（Panthera tigris）（n = 10）、丛林猫（Felis chaus）（n = 6）和家犬（Canis familiaris）（n = 4）的肺中检出成年吸虫。采用聚合酶链反应扩增ITS-2、部分28 SrDNA和部分cox1基因序列，并将这三个标记序列串联构建系统发育树。计算种内和种间遗传距离，利用部分cox1基因进行单倍型网络分析。结果基于连接树的系统发育分析表明，喀拉拉邦分离株与来自印度和其他国家的分离株形成了westermani假单胞菌复合体。喀拉拉邦分离株在遗传上与威氏假单胞虫形态2型更接近。然而，印度分离株的遗传距离较高，表明该复合体内的P. westermani之间存在显著的遗传变异。此外，印度也表现出最多的单倍型（n = 8）。结论对印度分离株的系统发育分析表明，它们可形成卫氏假单胞菌复合体。该寄生虫具有较高的遗传距离和大量的单倍型。需要进一步的流行病学研究来了解其遗传变异性。根据目前的分子数据，尚没有发现westermani的亚居群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.