Identification of pattern recognition receptors participating in the innate immune response to Tembusu virus

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-05-15 DOI:10.1007/s00705-025-06307-4

Jingjing Huang, Xiaoyang Hu, Duo Peng, Qiong Li, Yinuo Song, Dabing Zhang

引用次数: 0

Abstract

The pattern recognition receptors (PRRs) participating in the innate immune response to Tembusu virus (TMUV) were investigated using duck embryo fibroblasts (DEFs). TMUV infection induced significant upregulation of RIG-I and MDA5 and strong IFN-α, IFN-β, IL-6, and Mx responses. Overexpression of RIG-I or MDA5 resulted in increased IFN-α, IFN-β, IL-6, and Mx expression and decreased TMUV replication. Conversely, knockdown of RIG-I or MDA5 resulted in decreased IFN-α, IFN-β, IL-6, and Mx expression and increased TMUV replication. These findings demonstrate that RIG-I and MDA5 are essential sensors in the innate immune defense against TMUV, orchestrating cytokine production and restricting viral replication.

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参与坦布苏病毒先天免疫应答的模式识别受体的鉴定

利用鸭胚成纤维细胞（DEFs）研究了参与天布苏病毒（TMUV）先天免疫应答的模式识别受体（PRRs）。TMUV感染诱导rig - 1和MDA5的显著上调，以及强烈的IFN-α、IFN-β、IL-6和Mx反应。rig - 1或MDA5过表达导致IFN-α、IFN-β、IL-6和Mx表达增加，TMUV复制减少。相反，rig - 1或MDA5的敲低导致IFN-α、IFN-β、IL-6和Mx表达降低，并增加TMUV复制。这些发现表明rig - 1和MDA5在TMUV的先天免疫防御中是必不可少的传感器，协调细胞因子的产生和限制病毒复制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.