Metagenomics deciphers the evolution of microbial metabolic mechanisms at low temperatures: Focusing on cellular metabolism

IF 6.3 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of water process engineering Pub Date : 2025-05-17 DOI:10.1016/j.jwpe.2025.107945

Yin Wang , Taotao Tang , Qiang He , Xiaoliu Huangfu

{"title":"Metagenomics deciphers the evolution of microbial metabolic mechanisms at low temperatures: Focusing on cellular metabolism","authors":"Yin Wang , Taotao Tang , Qiang He , Xiaoliu Huangfu","doi":"10.1016/j.jwpe.2025.107945","DOIUrl":null,"url":null,"abstract":"<div><div>Prolonged operation times pose a major challenge for sewage treatment in alpine and high-altitude areas. This study investigates the impact of shortening the operating time at low temperature (4 °C) on nitrogen and phosphorus removal efficiency. Despite shortening the operating time from 10.5 h to 5 h, a removal efficiency of 98 % was achieved for chemical oxygen demand (COD), 99 % for NH<sub>4</sub><sup>+</sup>-N, 76 % for total nitrogen (TN), and 98 % for total phosphorus (TP). Shortening the operation time promoted microbial aggregation and activity by facilitating the secretion of proteins in extracellular polymer substances (EPS). The process of NO<sub>2</sub><sup>−</sup>-N reduction showed significant changes as the operation time was set to 8 h, which was attributed to the inhibition of electron transfer of complex III. Moreover, shortened operation times led to increased intracellular oxidative stress and cell membrane damage, thereby enhancing substance and electron transfer between microorganisms. Compared to direct contact, indirect contact between microorganisms was identified as a more stable pathway for electron transfer. This study provides a new perspective for optimizing the sewage treatment process in alpine and high-altitude areas.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"75 ","pages":"Article 107945"},"PeriodicalIF":6.3000,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214714425010177","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Prolonged operation times pose a major challenge for sewage treatment in alpine and high-altitude areas. This study investigates the impact of shortening the operating time at low temperature (4 °C) on nitrogen and phosphorus removal efficiency. Despite shortening the operating time from 10.5 h to 5 h, a removal efficiency of 98 % was achieved for chemical oxygen demand (COD), 99 % for NH₄⁺-N, 76 % for total nitrogen (TN), and 98 % for total phosphorus (TP). Shortening the operation time promoted microbial aggregation and activity by facilitating the secretion of proteins in extracellular polymer substances (EPS). The process of NO₂⁻-N reduction showed significant changes as the operation time was set to 8 h, which was attributed to the inhibition of electron transfer of complex III. Moreover, shortened operation times led to increased intracellular oxidative stress and cell membrane damage, thereby enhancing substance and electron transfer between microorganisms. Compared to direct contact, indirect contact between microorganisms was identified as a more stable pathway for electron transfer. This study provides a new perspective for optimizing the sewage treatment process in alpine and high-altitude areas.

查看原文本刊更多论文

宏基因组学解读低温下微生物代谢机制的进化：重点关注细胞代谢

长时间的运行对高山和高海拔地区的污水处理构成了重大挑战。本研究考察了在低温（4℃）下缩短操作时间对氮磷去除效率的影响。在将操作时间从10.5 h缩短至5 h的情况下，化学需氧量（COD）、氨氮（NH4+-N）、总氮（TN）和总磷（TP）的去除率分别达到98%、99%、76%和98%。缩短手术时间可以促进细胞外聚合物（EPS）中蛋白质的分泌，从而促进微生物的聚集和活性。当操作时间设置为8 h时，NO2−-N的还原过程发生了显著变化，这是由于配合物III的电子转移受到抑制。此外，缩短操作时间会增加细胞内氧化应激和细胞膜损伤，从而增强微生物之间的物质和电子传递。与直接接触相比，微生物之间的间接接触被认为是一种更稳定的电子转移途径。本研究为高寒高海拔地区污水处理工艺优化提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies