Quantitative detection and cellular imaging of hydrogen sulfide using a SERS probe based on AuAg nanocages

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-13 DOI:10.1016/j.bios.2025.117580

Xiaoli Wang , Jiale Zhang , Yun Wang, Yang Shen, Yuhan Zhang, Yuting Zhang, Nandi Zhou

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引用次数: 0

Abstract

Hydrogen sulfide (H₂S), a crucial gasotransmitter, plays an essential regulatory role in various physiological and pathological processes. There is an urgent need to develop sensitive and effective detection methods for intracellular H₂S, as abnormal H₂S levels are closely related to various diseases such as tumors. Herein, a surface-enhanced Raman scattering (SERS) probe was constructed for quantitative detection and imaging of H₂S in living cells. The SERS probe was obtained by using gold silver alloy nanocages (AuAg NCs) with superior plasmonic activity as SERS substrate, followed by modification with Raman signal molecule (4-ethynylaniline), mucin1 aptamer and polyethylene glycol. Upon exposure to H₂S, Ag in the SERS probe was rapidly and specifically converted to Ag₂S, leading to a remarkable decrease in the SERS intensity of the probe at 2010 cm⁻¹, a spectral region within the cell silent region. The developed SERS probe exhibited outstanding performances, including high sensitivity (with a detection limit as low as 0.36 nM for H₂S), remarkable selectivity, excellent stability and minimal cytotoxicity. Notably, this SERS probe had been successfully applied for the detection and imaging of both endogenous and exogenous H₂S levels in single living cells without bio-interference, highlighting its potential for precise and accurate intracellular H₂S monitoring. This advancement provides a powerful tool for studying H₂S-related physiological processes and disorders.

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基于AuAg纳米笼的SERS探针对硫化氢的定量检测和细胞成像

硫化氢（H2S）是一种重要的气体递质，在多种生理病理过程中起着重要的调节作用。细胞内H2S水平异常与肿瘤等多种疾病密切相关，迫切需要开发灵敏、有效的检测方法。本文构建了一种表面增强拉曼散射（SERS）探针，用于活细胞中H2S的定量检测和成像。SERS探针采用具有优异等离子体活性的金银合金纳米笼（AuAg NCs）作为SERS底物，然后用拉曼信号分子（4-乙基苯胺）、粘蛋白1适配体和聚乙二醇进行修饰。当暴露于H2S时，SERS探针中的Ag迅速特异性转化为Ag2S，导致探针的SERS强度在2010 cm−1（细胞沉默区的光谱区域）显著下降。所研制的SERS探针具有灵敏度高（对H2S的检出限低至0.36 nM）、选择性好、稳定性好、细胞毒性小等特点。值得注意的是，该SERS探针已成功应用于单个活细胞中内源性和外源性H2S水平的检测和成像，而不受生物干扰，突出了其精确和准确监测细胞内H2S的潜力。这一进展为研究h2s相关的生理过程和疾病提供了有力的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.