Epitope molecularly imprinted polymers based on host-guest interaction: specific recognition of CD59

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-05-14 DOI:10.1016/j.chroma.2025.466056

Yitong Xu , Huifeng Zhang , Ying Zhang , Jiutong Ma , Qiong Jia

{"title":"Epitope molecularly imprinted polymers based on host-guest interaction: specific recognition of CD59","authors":"Yitong Xu , Huifeng Zhang , Ying Zhang , Jiutong Ma , Qiong Jia","doi":"10.1016/j.chroma.2025.466056","DOIUrl":null,"url":null,"abstract":"<div><div>The detection of CD59 is of great clinical importance since it is an important glycoprotein that can serve as a biomarker related to kinds of cancers. In this work, we prepared host-guest interaction based oriented epitope molecularly imprinted polymer (hg-EMIP) for the immobilization of CD59 N-terminal epitopes. Cucurbit[7]uril (CB[7]) and l-phenylalanine (L-phe) were employed as the host and guest; and tannic acid (TA) with abundant hydroxyl groups and diethylenetriamine (DETA) were chosen as the functional monomer and crosslinking agent, respectively. The obtained hg-EMIP can specifically recognize CD59 with the adsorption capacity of 88.2 mg/g and imprinting factor of 5.63, and possesses high reusability. The hg-EMIP-based method possesses a wide linear range (1 ng/mL - 1 μg/mL) and low limit of detection (0.44 ng/mL), and can be successfully used for the detection of CD59 in human serum sample. This study provides a scheme for the preparation of host-guest based epitope molecularly imprinted polymers for helping to identify potential disease biomarkers efficiently.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1755 ","pages":"Article 466056"},"PeriodicalIF":3.8000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography A","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021967325004042","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

The detection of CD59 is of great clinical importance since it is an important glycoprotein that can serve as a biomarker related to kinds of cancers. In this work, we prepared host-guest interaction based oriented epitope molecularly imprinted polymer (hg-EMIP) for the immobilization of CD59 N-terminal epitopes. Cucurbit[7]uril (CB[7]) and l-phenylalanine (L-phe) were employed as the host and guest; and tannic acid (TA) with abundant hydroxyl groups and diethylenetriamine (DETA) were chosen as the functional monomer and crosslinking agent, respectively. The obtained hg-EMIP can specifically recognize CD59 with the adsorption capacity of 88.2 mg/g and imprinting factor of 5.63, and possesses high reusability. The hg-EMIP-based method possesses a wide linear range (1 ng/mL - 1 μg/mL) and low limit of detection (0.44 ng/mL), and can be successfully used for the detection of CD59 in human serum sample. This study provides a scheme for the preparation of host-guest based epitope molecularly imprinted polymers for helping to identify potential disease biomarkers efficiently.

查看原文本刊更多论文

基于主客体相互作用的表位分子印迹聚合物：CD59的特异性识别

CD59是一种重要的糖蛋白，可以作为与各种癌症相关的生物标志物，因此检测CD59具有重要的临床意义。在这项工作中，我们制备了基于主客体相互作用的定向表位分子印迹聚合物（hg-EMIP）用于固定CD59 n末端表位。以葫芦bb[7] （CB[7]）和l-苯丙氨酸（L-phe）为主客体；选择羟基丰富的单宁酸（TA）和二乙烯三胺（DETA）分别作为功能单体和交联剂。获得的hg-EMIP对CD59具有特异性识别能力，吸附量为88.2 mg/g，印迹因子为5.63，具有较高的可重复使用性。基于hg- emip的方法线性范围宽（1 ng/mL ~ 1 μg/mL），检出限低（0.44 ng/mL），可成功用于人血清样品中CD59的检测。该研究为制备基于主-客体表位的分子印迹聚合物提供了一种方案，有助于有效地识别潜在的疾病生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.