Single-step method for the immobilization of hydroxyapatite on 3D-printed porous polyetherketoneketone implants for the enhanced cell adhesion and osteogenic differentiation

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-14 DOI:10.1016/j.colsurfb.2025.114777

Semen Goreninskii , Igor Akimchenko , Alexander Vorobyev , Mikhail Konoplyannikov , Yuri Efremov , Evgeniy Sudarev , Peter Timashev , Andrei Zvyagin , Evgeny Bolbasov , Sergei Tverdokhlebov

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Abstract

Being biocompatible and extremely rigid polymer, polyetherketoneketone (PEKK) emerged as a promising material for the development of bone implants, but its inert surface limits bone-implant integration. Herein, we report a single-step approach for the immobilization of hydroxyapatite (HAp) particles on the surface of additively fabricated porous PEKK implants based on the swelling of the implant surface with subsequent entrapment of the HAp particles. By means of the scanning electron microscopy, it was established that this approach effectively preserved the morphology (pore diameter and printed line width) of the original implants. With that, up to 35.0 ± 14.0 % of the sample surface was covered with HAp particles, leading to improved hydrophilicity (<1° water contact angle). From the energy-dispersive spectroscopy results, calcium and phosphorus content on the surface of the modified samples reached 17.4 ± 4.1 wt% and 8.0 ± 1.7 wt%, respectively. Compression test revealed no changes in the samples strength. From the in vitro experiment with bone marrow multipotent stem cells (MSC) HAp immobilization improved cell adhesion (from 121 ± 40 cells/mm² to 234 ± 8 cells/mm²) and induced their osteogenic differentiation. Thus, the proposed method may be used for the development of PEKK-based implants for bone tissue restoration.

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羟基磷灰石单步固定在3d打印多孔聚醚酮酮植入物上，增强细胞粘附和成骨分化

聚醚酮酮（PEKK）是一种具有生物相容性和极高刚性的聚合物，是一种很有前途的骨植入材料，但其惰性表面限制了骨植入物的整合。在此，我们报道了一种单步固定羟基磷灰石（HAp）颗粒在增材制造的多孔PEKK种植体表面的方法，该方法基于种植体表面的膨胀和随后的HAp颗粒的夹带。扫描电镜结果表明，该方法有效地保留了原植入体的形态（孔径和印线宽度）。这样，高达35.0 ± 14.0 %的样品表面被HAp颗粒覆盖，从而提高了亲水性（<；1°水接触角）。从能谱分析结果来看，改性后样品表面的钙和磷含量分别达到17.4 ± 4.1 wt%和8.0 ± 1.7 wt%。压缩试验显示，试样的强度没有变化。骨髓多能干细胞（MSC）的体外实验表明，HAp固定能提高细胞粘附能力（从121 ± 40 cells/mm²增加到234 ± 8 cells/mm²），并诱导其成骨分化。因此，所提出的方法可用于开发基于pek的骨组织修复植入物。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.