A rapid and efficient microenvironment improving hyaluronic acid microneedle dressing for burn wound synergistic treatment

Abstract

Burn wounds are often accompanied by severe oxidative stress, sustained high expression of inflammation and solid bacterial biofilms formation. Therefore, it is urgent and important to develop strategies for effectively penetrate biofilms and rapidly regulate the burn pathological microenvironment. Herein, a multi-functional microneedle system was constructed for burn wounds synergistic treatment. Specifically, the nanocomposites HY@IND NCs with anti-inflammatory and antibacterial properties were obtained by co-assembling with solutol HS-15 (HS), ethyl lauroyl arginate hydrochloride (YG) and indomethacin (IND), which could be quickly ingested by bacteria or cells and had the characteristics of regulating the pathological microenvironment indicators for burns. It is then programmed to be loaded into a functional microneedle (MN) system along with the angiogenic drug deferoxamine (DFO) to obtain HY@IND-DFO MN. In vitro and in vivo experiments showed that it could not only effectively penetrate and destroy bacterial biofilms, but also the released HY@IND NCs could timely and effectively modulate the reactive oxygen species (ROS) and inflammatory microenvironment of the burn wounds as well as inhibit bacteria. More importantly, it could cooperate with DFO to promote wound regeneration and repair process. Briefly, this suitable microneedle drug delivery system may provide potential for the regeneration and repair of refractory wounds.