Electrochemical aptamer sensor for detection of SARS-CoV-2 S1 protein in real pharyngeal swab samples

IF 4.7 3区工程技术 Q2 ELECTROCHEMISTRY

Electrochemistry Communications Pub Date : 2025-05-15 DOI:10.1016/j.elecom.2025.107961

Yi Wang , Rui Bao , Xin Zhang , Chao Wang , Chunxia Song , Min Song , Hongjuan Jiang , Tan Wang , Xiangwei Wu , Zhaofeng Luo , Ying Lu

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引用次数: 0

Abstract

The global impact of the COVID-19 (Corona virus disease 2019) pandemic has been significant and the reliability of widely used diagnostic methods was often hindered by high false-negative rates as well as instrumentation limitations. In this context, electrochemical aptamer sensors can offer various advantages, including high sensitivity, low cost and the ability to perform detection directly on body fluids without the need for expensive equipments. In this study, a sensor was developed by hybridizing a SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2)-specific spike glycoprotein (S protein) aptamer with sulfhydryl- and ferrocene-labeled single-stranded DNA complementary to the aptamer. This design exhibited enhanced sensitivity compared with the labeled aptamer alone. Furthermore, the sensor's sensitivity, specificity and reproducibility were validated, and the results showed a linear relationship with the logarithm of the S1 protein concentration, which ranged from 10 fM to 100 nM (ΔI_p = 0.11 log C_{S1 protein} / fM - 0.021, R² = 0.99), with a LOD (limit of detection) of 1 fM (S/N = 3). Furthermore, the sensor successfully detected the S1 protein in pharyngeal swab samples from SAR S-CoV-2 patients. This study provides a promising approach for addressing public health crises and proposes a novel solution for managing infectious diseases during pandemics

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电化学适体传感器检测咽拭子样品中sars - cov - 2s1蛋白

COVID-19（2019冠状病毒病）大流行的全球影响巨大，广泛使用的诊断方法的可靠性往往受到高假阴性率和仪器限制的阻碍。在这种情况下，电化学适体传感器可以提供各种优势，包括高灵敏度、低成本和直接对体液进行检测的能力，而不需要昂贵的设备。在这项研究中，通过将SARS-CoV-2（严重急性呼吸综合征冠状病毒2）特异性刺突糖蛋白（S蛋白）适体与与该适体互补的巯基和二茂铁标记的单链DNA杂交，开发了一种传感器。与单独标记的适体相比，该设计显示出更高的灵敏度。结果表明，该传感器的灵敏度、特异性和重复性与S1蛋白浓度的对数呈线性关系，范围为10 fM ~ 100 nM （ΔIp = 0.11 log CS1蛋白/ fM - 0.021, R2 = 0.99），检出限为1 fM （S/N = 3）。此外，该传感器还成功检测了SAR S-CoV-2患者咽拭子样本中的S1蛋白。本研究为解决公共卫生危机提供了一种有希望的方法，并为大流行期间的传染病管理提出了一种新的解决方案

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来源期刊

Electrochemistry Communications 工程技术-电化学

CiteScore

8.50

自引率

3.70%

发文量

160

审稿时长

1.2 months

期刊介绍： Electrochemistry Communications is an open access journal providing fast dissemination of short communications, full communications and mini reviews covering the whole field of electrochemistry which merit urgent publication. Short communications are limited to a maximum of 20,000 characters (including spaces) while full communications and mini reviews are limited to 25,000 characters (including spaces). Supplementary information is permitted for full communications and mini reviews but not for short communications. We aim to be the fastest journal in electrochemistry for these types of papers.