Identification and characterization of a new strain of Ictalurid herpesvirus 1 isolated from channel catfish in China

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-05-13 DOI:10.1016/j.aquaculture.2025.742703

Fei Yu , Jiehua Xu , Sheng Yuan , Yi Yang , Siyang Song , Kai Hao , Rui Yuan , Yu Wang , Xiaohui Chen , Zhe Zhao

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引用次数: 0

Abstract

Ictalurid herpesvirus poses a significant threat to global catfish aquaculture. In this study, an Ictalurid herpesvirus 1 (IcHV1) strain, isolated from diseased channel catfish in China, was investigated. Initially, PCR assays using IcHV1 gene primers detected viral gene fragments in the tissues of diseased catfish. Meanwhile, transmission electron microscopy (TEM) confirmed the presence of herpesvirus-like virions. This virus, designated IcHV1-JS01, was isolated through incubation with CCO/BB cells, which was verified via PCR and immunoblotting; and infections with this virus triggered cytopathic effects and syncytia formation in CCO/BB cells. Subsequently, a catfish-challenge experiment proved its high virulence, with extremely high viral loads in dead fish. High-throughput sequencing of IcHV1-JS01 revealed a 133,530-bp genome, which shared high similarity with known viral genome, but harboured numerous variations. Phylogenetic analysis classified IcHV1-JS01 as a member of the genus Ictavirus, representing a unique Chinese isolate. Collectively, these findings will facilitate the development of epidemiological investigations and disease control strategies, protecting the catfish farming industry.

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中国渠道鲶鱼一株ⅰ型疱疹病毒的鉴定与特性分析

Ictalurid疱疹病毒对全球鲶鱼养殖业构成重大威胁。本研究对从中国病鲶中分离的一株Ictalurid herpesvirus 1 （IcHV1）进行了研究。最初，使用IcHV1基因引物进行PCR检测，检测出患病鲶鱼组织中的病毒基因片段。同时，透射电子显微镜（TEM）证实了疱疹病毒样病毒粒子的存在。该病毒命名为IcHV1-JS01，通过CCO/BB细胞孵育分离得到，并通过PCR和免疫印迹进行验证；感染这种病毒会引发CCO/BB细胞的细胞病变和合胞体形成。随后，一项鲶鱼挑战实验证明了它的高毒性，在死鱼中具有极高的病毒载量。高通量测序显示，IcHV1-JS01基因组全长133,530 bp，与已知病毒基因组高度相似，但存在大量变异。系统发育分析表明，IcHV1-JS01为伊坦病毒属成员，是一种独特的中国分离株。总的来说，这些发现将促进流行病学调查和疾病控制战略的发展，保护鲶鱼养殖业。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.